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N-acetylcysteine: pharmacological considerations and experimental and clinical applications.
It is hoped that the experience gained with this unique agent will help in future efforts to design antioxidants and chemoprotective principles which are able to more accurately utilize endogenous biochemical processes for cell- or tissue-specific therapy. Expand
Identification of S-glutathionylated cellular proteins during oxidative stress and constitutive metabolism by affinity purification and proteomic analysis.
The ability to chemically tag, select, and identify S-glutathionylated proteins, particularly during constitutive metabolism, will greatly enhance efforts to establish posttranslational redox modification of cellular proteins as an important biochemical control mechanism in coordinating cellular function. Expand
S-thiolation of human endothelial cell glyceraldehyde-3-phosphate dehydrogenase after hydrogen peroxide treatment.
It is shown that the post-translational modification of the cysteinyl thiols of glyceraldehyde-3-phosphate dehydrogenase accompanies an inhibition of the enzyme and that both events are simultaneously and rapidly reversed upon the removal of the oxidative stimulus. Expand
Methodologies for the application of monobromobimane to the simultaneous analysis of soluble and protein thiol components of biological systems.
A series of simple methodologies for the determination of the redox status of low molecular weight and protein thiols in biological systems is described. Based centrally upon the use ofExpand
Expression of drug metabolizing enzymes in hepatocyte-like cells derived from human embryonic stem cells.
This first report on expression of all major CYPs in hepatocyte-like cells derived from hESC represents an important step towards functional hepatocytes, but efforts to further differentiate the cells using optimized protocols are needed before they exhibit similar levels of drug metabolizing enzymes as primary human hepatocytes and liver. Expand
Rapid and Specific Efflux of Reduced Glutathione during Apoptosis Induced by Anti-Fas/APO-1 Antibody*
Stimulation of GSH efflux provides a novel mechanism whereby Fas/APO-1 ligation can deplete GSH and enhances the oxidative tonus of a responding cell without requiring an increase in the production of reactive oxygen species. Expand
Studies on the mechanism of oxidative modification of human glyceraldehyde-3-phosphate dehydrogenase by glutathione: catalysis by glutaredoxin.
The data indicate the complexity of mechanisms likely to be involved in regulating cellular proteins during oxidative stress and implicate controlled enzyme-catalysed S-glutathionylation as a potential selectivity factor in the redox modification of protein function by glutathione. Expand
Hydrogen peroxide causes greater oxidation in cellular RNA than in DNA
Human A549 lung epithelial cells were challenged with 18O-labeled hydrogen peroxide and showed slow turnover rates of adducts in RNA and DNA, where guanine in RNA was 14–25 times more common than in DNA. Expand
Isoforms of alanine aminotransferases in human tissues and serum--differential tissue expression using novel antibodies.
The development of novel isoenzyme specific ALT1 and ALT2 antibodies and the expression of the enzymes in human cells and organs are described and substantiate a need for investigations regarding the possible impacts on ALT measurements. Expand