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Emesis induced by inhibitors of type IV cyclic nucleotide phosphodiesterase (PDE IV) in the ferret
TLDR
The results suggest that the PDE IV inhibitors studied are mixed peripheral-central emetogens, and whether emesis is mediated via a specific isoform of PDEIV remains to be established. Expand
Metabolism of U6 RNA species in nonirradiated and UV‐irradiated mammalian cells
TLDR
It is suggested that UV radiation induces the specific degradation of the electrophoretically faster moving species of U6 RNA, which are apparently shorter chains. Expand
Inhibition of HIV-1 replication by a Tat RNA-binding domain peptide analog.
TLDR
The results suggest that a peptide or peptide mimetic capable of competing with the TAR RNA-binding domain of Tat protein might be useful as a therapeutic agent for AIDS. Expand
Use of a polyethylene glycol-peptide conjugate in a competition gel shift assay for screening potential antagonists of HIV-1 Tat protein binding to TAR RNA.
TLDR
An electrophoretic assay to screen potential low-molecular-weight (< 2 kDa) Tat antagonists has been established and determines the ability of a potential antagonist to compete with Tat-PEG for binding to Delta TAR, as measured by interference with the gel shift of delta TAR. Expand
Effect of ultraviolet light on the expression of genes for human U1 RNA
TLDR
U1 RNA transcription in isolated nuclei from HeLa cells, assayed by RNAase T1 protection, reproduced the delayed inhibition of small nuclear RNA species U1, U2, U3, U4, and U5, suggesting that these cells may produce small amounts of U1 RNA molecules with variant nucleotide sequences in the mature region of the transcript. Expand
Translational effects of peptide antagonists of Tat protein of human immunodeficiency virus type 1.
TLDR
While the Tat antagonist greatly reduces CAT protein production and polysome association of CAT mRNA, it has little effect on CAT mRNA levels, suggesting that the antagonist works at the post-transcriptional level. Expand
A polyethylene glycol copolymer for carrying and releasing multiple copies of cysteine-containing peptides.
TLDR
To illustrate the potential biomedical usefulness of this polymer carrier, a Tat peptide-PEG conjugate was shown to inhibit expression of a reporter gene fused to the TAR element of human immunodeficiency virus in a model cell assay. Expand
Metabolism of small RNAs in cultured human cells
TLDR
The level of radiolabeled mature U1 RNA doubled between 2 and 24 hr of label chase, while that of all other small RNA species tested did not change, compatible with the possibility that the nucleotide precursor pool for U1 DNA transcription may be partly segregated, or that there may be a second pathway of U1RNA formation. Expand