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Production of l-Phenylalanine from trans-Cinnamic Acid with Rhodotorula glutinis Containing l-Phenylalanine Ammonia-Lyase Activity
TLDR
The enzyme activity was kept stable for a relatively long time during cultivation by the addition of l-isoleucine and may provide a rapid and practical way to produce l-phenylalanine useful as an essential amino acid. Expand
Glycolytic pathway as an ATP generation system and its application to the production of glutathione and NADP
Efficient ATP generation is required to produce glutathione and NADP. Hence, the generation of ATP was investigated using the glycolytic pathway of yeast. Saccharomyces cerevisiae cells immobilizedExpand
Basic studies for continuous production of L-aspartic acid by immobilized Escherichia coli cells.
TLDR
Conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice and the immobilized cell column was very stable. Expand
Continuous production of L‐citrulline by immobilized Pseudomonas putida cells
TLDR
The microbial cells of Pseudomonas putida ATCC 4359 were immobilized by entrapment in a polyacrylamide gel lattice and the permeability of substrate or product through the cell wall und the heat stability of the enzyme were increased by immobilization of the cells. Expand
Biosynthesis of norvaline, norleucine, and homoisoleucine in Serratia marcescens.
TLDR
Norvaline and homoisoleucine are considered to be formed fromalpha-ketobutyrate and alpha-keto-beta-methylvalerate by the leucine biosynthetic enzymes, and this view was confirmed by the findings that a norvaline accumulator could be obtained by derivation of theLeucine accumulators into an isoleucin-valine auxotroph. Expand
Enzymatic production of urocanic acid by Achromobacter liquidum.
TLDR
Yields of isolated urocanic acid of over 92% from L-histidine were easily attainable and when the culture of Achromobacter liquidum was added to DL- histidine, D-histazine and uro canic acid were simultaneously obtained in high yields. Expand
Immobilized aspartase-containing microbial cells: preparation and enzymatic properties.
The immobilization of asparatase-containing Escherichia coli was investigated by various methods, and the most active immobilized cells were obtained by entrapment in a polyacrylamide gel lattice.Expand
Immobilization of enzymes and microbial cells using carrageenan as matrix
TLDR
Conditions for the gelation of k ‐carrageenan, which is a new polymer for immobilization of enzymes and microbial cells, were investigated in detail and immobilized preparations were easily tailor‐made to various shapes such as cube, bead, and membrane. Expand
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