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Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid ω-hydroxylation in development
TLDR
It is indicated that LCR-dependent ω-hydroxylation of fatty acids could be implicated in the biosynthesis of cutin in the epidermis and in preventing postgenital organ fusions. Expand
CYP86A1 from Arabidopsis thaliana encodes a cytochrome P450-dependent fatty acid omega-hydroxylase.
TLDR
Genomic organization analyzed by Southern blot suggested a single gene encoding CYP86A1 in A. thaliana, which was found to catalyze the omega-hydroxylation of saturated and unsaturated fatty acids with chain lengths from C12 to C18 but not of hexadecane. Expand
CYP86B1 Is Required for Very Long Chain ω-Hydroxyacid and α,ω-Dicarboxylic Acid Synthesis in Root and Seed Suberin Polyester1[W][OA]
TLDR
It is demonstrated that CYP86B1 is a very long chain fatty acid hydroxylase specifically involved in polyester monomer biosynthesis during the course of plant development. Expand
CYP94A5, a new cytochrome P450 from Nicotiana tabacum is able to catalyze the oxidation of fatty acids to the omega-alcohol and to the corresponding diacid.
TLDR
A cytochrome P450 able to catalyze the complete set of reactions oxidizing a terminal methyl group to the corresponding carboxyl is described, which is enantioselective: after incubation of a synthetic racemic mixture of 9,10-epoxystearic acid, the chirality of the residual epoxide was 40/60 in favor of 9R,10S enantiomer. Expand
Functional expression in yeast and characterization of a clofibrate-inducible plant cytochrome P-450 (CYP94A1) involved in cutin monomers synthesis.
TLDR
The chemical tagging of a cytochrome P-450-dependent lauric acid omega-hydroxylase from clofibrate-treated Vicia sativa seedlings with [1-14C]11-dodecynoic acid allowed the isolation of a full-length cDNA designated CYP94A1, which was strong regioselectivity shifted with sulphur-containing substrates. Expand
Cytochrome P450-dependent fatty acid hydroxylases in plants
TLDR
The best characterized fatty acid hydroxylases are presented and their possible roles in plant defense and development, fatty acid catabolism, plant reproduction and detoxification are discussed. Expand
Purification and characterization of the NADPH-cytochrome P-450 (cytochrome c) reductase from higher-plant microsomal fraction.
TLDR
Substrate saturation curves as a function of NADPH at fixed concentration of cytochrome c are compatible with a sequential type of substrate-addition mechanism and the enzyme was able to reconstitute cinnamate 4-hydroxylase activity when associated with partially purified tuber cy tochrome P-450 and dilauroyl phosphatidylcholine in the presence of NADH. Expand
Cloning and functional characterization of CYP94A2, a medium chain fatty acid hydroxylase from Vicia sativa.
TLDR
Heterologous expression of CYP94A2 in Saccharomyces cerevisiae yeast strain WAT11 shows that it catalyses the hydroxylation of myristic acid with a K(m(app)) of 4.0 microM and a turnover rate number of 80 min(-1). Expand
Localization of CYP86B1 in the outer envelope of chloroplasts.
TLDR
This study provides the first direct evidence for a chloroplast-localized cytochrome P450-dependent monooxygenase in pea chloroplasts. Expand
Evolutionary relationship and substrate specificity of Arabidopsis thaliana fatty acid omega-hydroxylase
TLDR
Evidence that substrate specificity is conserved in phylogenetically related isoforms is provided and suggests specific residues in the active site pocket that play a key role in determining substrate specificity. Expand
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