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Vascular–Platelet and Plasma Hemostasis Regulators from Bloodsucking Animals
TLDR
Saliva of bloodsuckers (leeches, insects, ticks, vampire bats) contains various regulators of some hemostatic stages, which are summarized in this review. Expand
Genes from the medicinal leech (Hirudo medicinalis) coding for unusual enzymes that specifically cleave endo-ɛ(γ-Glu)-Lys isopeptide bonds and help to dissolve blood clots
TLDR
The first purification of destabilase, part of its amino acid sequence, isolation and sequencing of two related cDNAs derived from the gene family that encodes destabilase proteins, and the detection of isopeptidase activity encoded by one of these c DNAs cloned in a baculovirus expression vector are reported. Expand
Destabilase from the medicinal leech is a representative of a novel family of lysozymes.
TLDR
New proteins homologous to destabilase were recently described for Caenorhabditis elegans and bivalve mollusks suggesting that the new lysozyme family can be widely distributed among invertebrates and it remains to be investigated whether the two enzymatic activities are attributes of one and the same protein. Expand
Destabilase, the novel epsilon-(gamma-Glu)-Lys isopeptidase with thrombolytic activity.
The salivary gland secretion of the leech Hirudo medicinalis contains the enzyme destabilase which hydrolyses epsilon-(gamma-Glu)-Lys cross-links in stabilized fibrin. Accumulation of Glu residuesExpand
Proteinase Inhibitors from the Medicinal Leech Hirudo medicinalis
TLDR
The medicinal leech Hirudo medicinalis produces various types of proteinase inhibitors that are inhibitor of factor Xa, hirudin (thrombin inhibitor), inhibitor of carboxypeptidase, and inhibitor of complement component C1s. Expand
Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis
TLDR
Comparison of detected molecular masses with those of six known biologically active compounds produced by medicinal leeches revealed their uneven distribution in SGS of each of the three leech species, which opens prospects for using certain species of medicinal leech for targeted therapy of various pathologies. Expand
Protein Profiling of the Medicinal Leech Salivary Gland Secretion by Proteomic Analytical Methods
Protein diversity of the high molecular weight fraction (molecular mass > 500 daltons) of salivary grand secretion of the medicinal leech Hirudo medicinalis has been demonstrated using methods ofExpand
Enzyme from the medicinal leech (Hirudo medicinalis) that specifically splits endo‐ϵ(‐γ‐Glu)‐Lys isopeptide bonds: cDNA cloning and protein primary structure
TLDR
The isolation and sequence peculiarities of the third cDNA of the family as well as the complete sequence of the destabilase protein are reported, finding that the inferred mature protein product of this cDNA matches the independently determined destabilaseprotein sequence. Expand
Antibacterial Non-Glycosidase Activity of Invertebrate Destabilase-Lysozyme and of Its Helical Amphipathic Peptides
TLDR
DL is the first invertebrate lysozyme with combined enzymatic and non-enzymatic antibacterial action for destabilase-lysozyme from medicinal leech. Expand
Genes from the medicinal leech (Hirudo medicinalis) coding for unusual enzymes that specifically cleave endo-epsilon (gamma-Glu)-Lys isopeptide bonds and help to dissolve blood clots.
TLDR
The first purification of destabilase is reported, part of its amino acid sequence isolation and sequencing of two related cDNAs derived from the gene family that encodes destabilase proteins, and the detection of isopeptidase activity encoded by one of these c DNAs cloned in a baculovirus expression vector. Expand
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