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Secretion of functional human enzymes by Tetrahymena thermophila
TLDR
The ciliate expression system has the potential to become an excellent expression system, however, additional optimisation steps including host strain improvement and measures to increase the yield of expression are necessary to be able to provide an alternative to the common E. coli and yeast-based systems as well as to transformed mammalian cell lines.
A Novel Malaria Vaccine Candidate Antigen Expressed in Tetrahymena thermophila
TLDR
This study has successfully demonstrated the use of Tetrahymena thermophila as a recombinant protein expression platform for the production of malaria vaccine antigens.
Biochemical and molecular characterisation of Tetrahymena thermophila extracellular cysteine proteases
TLDR
The information provided will allow silencing of protease activity by either knock out methods or by Tetrahymena specific antisense-ribosome-techniques and will facilitate the next step in the advancement of this exciting organism for recombinant protein production.
Expression, secretion and surface display of a human alkaline phosphatase by the ciliate Tetrahymena thermophila
TLDR
The fact that ciliates do not secrete an endogenous alkaline phosphatase provides the possibility to use the truncated hiAP as a reporter enzyme, allowing the quantification of measures that will be necessary for further optimization of the host strains and the fermentation processes.
The stoichiometry of subunit c of Escherichia coli ATP synthase is independent of its rate of synthesis.
TLDR
Data clearly demonstrate that the stoichiometry of the subunit c rings remains constant even after reduction of the synthesis of sub unit c.
Novel ciliate lipases for enzyme replacement during exocrine pancreatic insufficiency
TLDR
Three novel ciliate lipases derived from Tetrahymena thermophila are cloned and analyzed and are promising candidates for an improved enzyme replacement therapy for exocrine pancreatic insufficiency.
Long term follow-up of a simplified and less burdened pancreatic duct ligation model of exocrine pancreatic insufficiency in Goettingen Minipigs
TLDR
Pancreatic duct ligation in minipigs is an excellent method of inducing exocrine pancreatic insufficiency and has the potential to be used in long-term EPI studies with up to 100 weeks post-OP without supplementation with enzymes and vitamins.
Long - term follow up of a simplified and less burdened pancreatic duct ligation model of exocrine pancreatic insufficiency in Goettingen Minipigs
BackgroundPancreatic duct ligation in a minipig model leads to exocrine pancreatic insufficiency (EPI). This allows studies of digestive processes and pancreatic enzyme replacement therapies