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Efficient transposition in mycobacteria: construction of Mycobacterium smegmatis insertional mutant libraries.
- C. Guilhot, I. Otal, I. Van Rompaey, C. Martín, B. Gicquel
- Biology, Medicine
- Journal of bacteriology
The Tn611 transposon was inserted into pCG63, a temperature-sensitive plasmid isolated from an Escherichia coli-mycobacterial shuttle vector which contains the pAL5000 and pUC18 replicons. The… Expand