I. V. Skachkov

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The dynamics of coated microbubbles was studied in an in vivo model. Biotinylated lipid-coated microbubbles were prepared in-house and were injected into a chick embryo chorioallantoic membrane (CAM) model on the fifth day of incubation. The microbubbles, ranging between 1.0 and 3.5 μm in diameter, were insonified in the frequency range of 4-7 MHz. Two(More)
A comparison between phospholipid-coated microbubbles with and without liposomes attached to the microbubble surface was performed using the ultra-high-speed imaging camera (Brandaris 128). We investigated 73 liposome-loaded microbubbles (loaded microbubbles) and 41 microbubbles without liposome loading (unloaded microbubbles) with a diameter ranging from(More)
Ultrasound molecular imaging visualizes disease biomarkers using targeted ultrasound contrast agents (t-UCA). Different lipids result in UCA with different acoustic behavior. In this study, subharmonic (SH) response of MicroMarker UCA and four homemade UCA with different lipid coatings were compared in vitro with a high frequency ultrasound system (Vevo(More)
Microbubbles (MBs) have been shown to create transient or lethal pores in cell membranes under the influence of ultrasound, known as ultrasound-mediated sonoporation. Several studies have reported enhanced drug delivery or local cell death induced by MBs that are either targeted to a specific biomarker (targeted microbubbles, tMBs) or that are not targeted(More)
Fetal bovine serum has been shown to considerably inhibit phagocytosis of non-opsonized 2-μm fluorescent latex beads by cultured macrophages IC-21. Phagocytic activity was assessed using fluorescent microscopy and specially devised ImageJ plugins. Phagocytosis percent, PP (percentage of the bead-containing cells in the cell population under study), and(More)
In vivo cell tracking is an emerging field and a promising technique for tracking stem cells, metastasis and inflammation. The aim of this study was to find optimal US parameters for ultrasound contrast agent-mediated SPIO-labeling of endothelial cells for MRI tracking. The optimal ultrasound parameters were 40 kPa at 1 MHz, 10,000 cycles, 20 Hz pulse(More)
Angiogenesis is a critical feature of plaque development in atherosclerosis and might play a key role in both the initiation and later rupture of plaques. The precursory molecular or cellular pro-angiogenic events that initiate plaque growth and that ultimately contribute to plaque instability, however, cannot be detected directly with any current(More)
Ultrasound contrast agents as drug delivery systems are an emerging field. Recently, it was shown that targeted microbubbles (tMB) are able to sonoporate endothelial cells in vitro. This study investigates if tMB can also induce sonoporation in vivo, thereby making it possible to combine molecular imaging and drug delivery. Live chicken embryos were chosen(More)
Previously we have shown that a suitable design of the excitation envelope to generate a self-demodulation (SD) signal at the subharmonic (SH) frequency can enhance the SH emission of ultrasound contrast agent (UCA) more than 20 dB at 10 MHz transmit frequency. The SH emission from the UCA is of interest since it is produced only by the UCA and is free of(More)
The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is the low number of cells delivered at the area at risk. To improve the delivery, we are currently investigating a novel delivery platform in which stem cells are conjugated with targeted(More)