I V Domaradskij

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The military-biological complex of the former Union of Soviet Socialist Republics was a true Frankenstein's Monster, with a powerful scientific potential--or good and for ill. This article examines both the direct scientific results of the twin biological weapons (BWs) programmes run by the 'civilian' Biopreparat and by the Ministry of Defence (MoD) and the(More)
On the base of plasmid pLD720 (a deletion derivative of the cosmid vector pHC79) a number of hybrid plasmids which confer in Escherichia coli cells the kanamycin resistance was constructed. All hybrid plasmids contain the promoterless part of kanamycin resistance gene (which codes for aminoglycoside 3'-phosphotransferase II) from transposon Tn5. The Km gene(More)
It was shown that the transcription initiation of the kanamycin resistance gene (the Km gene) from transposon Tn5 in E.coli HB101 strain can be provided by specific yeast sequences. To localize a region of the yeast DNA involved in transcription initiation, a number of hybrid plasmids containing promoterless part of the Km gene fused with DNA sequences from(More)
A hybrid plasmid capable of replication in 2 different genera, Escherichia and Pseudomonas, was constructed. This plasmid DNA can be used as a cloning vector in E. coli and pseudomonades cells. The described hybrid plasmid pLD411 has been constructed on the basis of 2 small E. coli vector R-plasmids used in our laboratory and cryptic plasmid pWW2 or P.(More)
A number of hybrid plasmids which can autonomously replicate in E. coli, B. subtilis and S. cerevisiae was constructed. Replication of these plasmids both in yeast and in B. subtilis starts on a sequences originating from Staphylococcus aureus plasmids pC194 and pE194. In yeast these hybrids are unstable like those yeast vectors which contain eukaryotic(More)
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