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Potential-gated currents in the membranes of enzymatically isolated neurons from the lamprey spinal cord were investigated using the whole cell variant of the patch-clamp technique. As it was revealed the main currents underlying action potential (AP) in the dorsal sensory cells as well as in branched cells are inward Na(+)- and outward K(+)-currents.… (More)
Patch-clamp recordings in a whole-cell mode were performed on dorsal sensory cells enzymatically isolated from the spinal cord of two lamprey species, Ichthyomyzon unicuspis and Lampetra fluviatilis. The voltage-activated currents through calcium channels were analysed. GABA and the specific GABA(B) receptor agonist baclofen reduced the peak amplitude of… (More)
GABA and GABAB receptor agonists were shown to reduce the peak calcium current amplitude with its subsequent recovery, whereas glycine and taurine, the GABAA receptor agonists, did not modify the current. The findings suggest that the GABAB receptors mediate a presynaptic inhibition by suppression of the Calcium currents in the cyclostome spinal cord.
Effects of inhibitory mediators on the membranes of isolated lamprey spinal cord neurones were investigated by means of whole-cell recording and concentration clamp techniques. Glycine and gamma-aminobutyric acid (GABA) applications evoked desensitizing chloride currents. The concentrations at half-maximum effects were 16 microM for glycine- and 1.5 mM for… (More)
Synaptic responses in motoneurons in the isolated spinal cord of the lamprey during stimulation of the reticulospinal axons were examined. Chemical transmission in the synapses was partially or completely blocked by temperature reduction of the perfusing solution, pentobarbitone application or substitution of Mn2+ ions for Ca2+ in the perfusate. Excitatory… (More)