The conditions for maintaining boar type A spermatogonia in culture are optimized. An RT PCR analysis of mRNA of Nanog, PLZF, POU5F1, and VASA genes with hybridization fluorescence detection showed that the conditions of culturing boar spermatogonial cells affect the expression of these genes.
We studied the in vitro effect of Sertoli cells on boar spermatogonia isolated from the testes of 60-day-old crossbred boars. In order to enrich the culture with spermatogonia, the cells were purified by density gradient centrifugation with the use of Percoll gradient followed by separation based on adhesive capacities of cells. We found lipid drops stained… (More)
Integrin expression during long-term cultivation in multipotent mesenchymal stromal cells (MMSCs) isolated from human subcutaneous adipose tissue was studied. It was found that expression of α1, α4 and α6 integrins at the 2nd and 17th passages was different. The cell number positively stained with antibodies against CD49a (α1 integrin) and CD49d (α4… (More)