I. P. Ermakov

Learn More
The involvement of Cl− in cytoplasm polarization in the pollen tube and membrane potential control during pollen germination in vitro was studied by fluorescence techniques in Nicotiana tabacum. Cl− release from cells was blocked by the anion channel inhibitor nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) or by the addition of Cl− to the incubation(More)
Nonaqueous titration was used for detection of free amino groups in the polymeric matrix of plant cell walls. The content of amino groups varied in the range 0.54–0.91 and total nitrogen in the range 1.0–4.2 mmol per gram dry mass of cell walls depending on the plant species. However, these data on the high content of free amino groups do not correlate with(More)
The formation of reactive oxygen species in pollen at the early germination stage, which precedes the formation of the pollen tube, was studied. During this period, pollen grain is being hydrated, abruptly increasing its volume, and it passes from the resting state to active metabolism. Fluorescent methods have made it possible to reveal reactive oxygen(More)
In order to determine the role of the plasma membrane H(+)-ATPase and alternative oxidase (alternative pathway of respiration) in the regulation of intracellular pH during development of the tobacco male gametophyte, we studied the changes in pH due to the inhibition of these enzymes by orthovadanate and benzhydroxamic acid, respectively. The inhibition of(More)
The regulatory role of intracellular pH changes and of transmembrane Cl– transport in the activation of Nicotiana tabacum L. pollen grains at a stage preceding in vitro germination was studied. The acidification of the cytosol with propionic acid hindered the germination of pollen grains, whereas its alkalization by fusicoccin-stimulated H+-ATPase activity(More)
In order to determine the role of the plasma membrane H+-ATPase and alternative oxidase (alternative pathway of respiration) in the regulation of intracellular pH during development of the tobacco male gametophyte, we studied the changes in pH due to the inhibition of these enzymes by orthovadanate and benzhydroxamic acid, respectively. The inhibition of(More)
We studied the dynamics of mobile potassium, chloride, and nitrate ions during development of the micro-spore and differentiation of the pollen grain in Nicotiana tabacum L. by measuring their concentration in aqueous extracts from cells destroyed by freezing-thawing using ion-selective electrodes. Stage-specific changes in the ion content and intracellular(More)
Compared to lower plants and animals, the study of early embryogenesis in higher plants is associated with considerable difficulties. Quite a few studies are devoted to the assessment of the levels of endogenous hormones at different embryogenetic stages; however, most of the data available concern the postglobular stages of embryonic development, at which(More)
Changes in intracellular pH, K+and Cl–concentrations were measured in pollen grains, anther loculi, and whole anthers during in vivodifferentiation of male gametophyte of Nicotiana tabacumL. The effects of extracellular K+and Cl–on intracellular pH regulation were also studied during development of pollen grains in vitro. Ion-selective electrodes and X-ray(More)