I. Pšenička

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The effect of twelve detergents on aspartate ammonia-lyase activity ofEscherichia alcalescens used for the production of L-aspartic acid was tested. Best permeabilization was found with Triton X-100, Slovafol 910 and Corona, a mixed commercial preparation. In contrast to Triton X-100 and Slovafol 910, a much narrower range of suitable concentrations was(More)
Aspartate ammonia-lyase activity ofEscherichia alcalescens was positively affected by the composition of the culture medium and by a higher intensity of aeration. By using the supernatant obtained from autolyzed baker’s yeast, the aspartate ammonia-lyase activity increased two-fold (about 90 μkat/g wet biomass) as compared to commercial yeast autolyzates.
A new selection method of yeast cell hybrids, based on the application of yeast killer factors, has been developed in the model system of induced protoplast fusion of Saccharomyces cerevisiae superkiller strain T158C (alpha, his-) and different sensitive strains. The method is comparable in its efficiency to the currently used selection techniques based on(More)
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