I O Bogoliubova

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Within the framework of studying the "2-cell block in vitro" phenomenon, two variants of inhibitory-induced stoppage of development at a two-cell stage were produced and analysed. Mimosine arrested the cleavage on the G1/S interface, and genistein at G2 stage of the second cell cycle. In the experimentally blocked embryos a detailed study was made of the(More)
The distribution of pre-mRNA splicing factors (snRNPs and SR-protein SC35) was studied in the nuclei of early and late 2-cell mouse embryos using light and electron immunocytochemistry. The splicing factors were shown to be present in the nuclei before the beginning of embryonic transcription. In inactive nuclei of early 2-cell embryos, SR-protein SC35 is(More)
Nuclear distribution of unphosphorylated and phosphorylated forms of RNA polymerase II in two celled mouse embryos was studied using immunoelectron microscopy. RNA polymerase II was shown to be present in the karyoplasm of mouse embryo nuclei at the early two-celled stage, that is before activation of the embryonic genome. During this period, RNA polymerase(More)
In this study, preimplantation mouse embryos were used as a new model for investigation of actin distribution in the nuclei and identification of functional forms of intranuclear actin. Combination of direct detection of actin by fluorescent-conjugated falloidin and DNase I with the method of indirect immunofluorescence was applied as an integrated approach(More)
Vital observation in combination with electron microscopy and immunocytochemistry was used for studying structural organization and developmental potential of BALB/c mouse embryos after cleavage cessation at a two cell stage caused by the "two-cell block in vitro" phenomenon. Modification of structure and viability of embryos was followed for 2 days from(More)
Effects of a few pharmacological agents (ouabain, proserine, atropine) upon absorption velocity in intestinal epithelium, were studied in lower vertebrates. Mechanisms of the intestinal epithelium response to osmotic irritants and regulation of this process by the enteric portion of the metasympathetic nervous system, are discussed.
In this work, new data on the peculiarities of actin immunofluorescent detection in 2-cell mouse embryos using antibodies against C- and N-terminal domains are presented. We studied the distribution of nuclear actin after artificial suppression of transcription and after enzymatic digestion of DNA. The visual observations were supplemented with morphometric(More)
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