I M Tarasova

  • Citations Per Year
Learn More
A vector plasmid directing the synthesis of antisense RNA of Escherichia coli gene htpR has been constructed. For this fragment of htpR gene encoding the synthesis of N-terminal sequence of HtpR protein has been cloned in this plasmid in the opposite orientation under the control of Pr promoter of bacteriophage lambda. Under conditions of Pr transcription(More)
A new vector has been constructed on the basis of plasmid pCQV2 containing thermoinducible regulatory elements of bacteriophage lambda. The vector makes it possible to combine an inducible synthesis of foreign proteins with negative control of intracellular proteolysis. The principle employed for the construction of the plasmid implies the regulation of(More)
Present work shows that changes in the surface potential (SP) are linearly connected with alterations of the cell membrane potential (MP) at three physiological states of the organism: control (C), induced cancerogenesis (IC) and total reaction of the organism to damage (TRD). In the control greater SP changes are corresponded with the least MP changes.(More)
Electrophoretic mobility of murine lymphocytes of the spleen was investigated during the development of reaction to damage and to the initial stages of induced cancerogenesis. These two processes differ in kinetics data of the change of electrophoretic mobility. A correlation was found between the electrophoretic mobility and ability of the cell for blast(More)
The mechanisms of induction of heat shock protein synthesis in E. coli have been studied. For this purpose plasmids in which htpR gene expression is controlled by the PR-promoter of bacteriophage lambda and by the Trp-promoter have been constructed. An effective induction of heat shock proteins requires both an increased content of htpR protein and(More)
A thermoresistant htpR mutant having a decreased level of proteolytic activity has been selected in E. coli strain K802 after the directed mutagenesis in vivo. The mutation results in the bacteriophage T7 RNA-polymerase stability, aminoglycosidephosphotransferase stability as well as in the decrease in the rate of proteolytic degradation of cytoplasmic(More)
It has been established that the plasmid encoding the N-terminal domain of RecA protein (50 amino acids residues) disturbs SOS functions of htpR- mutants of E. coli. This is expressed in increased UV-sensitivity of strains and in the poor transcription of the aminoglycosidephosphoransferase gene in the hybrid operon recA APT. The discovered effect is due to(More)
  • 1