I. M. G. Padilla

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We have designed an innovative construct (pX6-DAO1) combining the chemical inducible Cre-LoxP system and the conditional selectable marker gene dao1 to obtain marker-free transgenic tobacco plants. Nicotiana tabaccum transgenic lines were regenerated on medium with 6 mM d-alanine. The DNA site-specific recombination was controlled by the inducer(More)
Cultivated asparagus (Asparagus officinalis L.) is an economically important plant worldwide. “Morado de Huetor” is a Spanish autochthonous landrace characterized by their longevity, organoleptic characteristics, differential biocompound content and high heterozygosity, resulting in heterogeneous plantations with limited productivity. Consequently, this(More)
Plum pox virus (PPV), a quarantine virus, is the causal agent of sharka, the most devastating viral disease of stone fruits. The aim of this study was to produce several transgenic plum lines resistant to PPV, set up an efficient and reliable in vitro PPV resistant test and compare it with the standard greenhouse evaluation. Transgenic plants with the(More)
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