I M Antonikov

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Platelets were stimulated in vitro by different ADP concentration (10(-7)--10(-9) M). Scanning electron microscopy, light transmission recording and the Ca-sensitive fluorescent probe, chlortetracycline, were used to study the time course of changes in the shape, aggregation and the amount of membrane-bound calcium during ADP activation. It was found that(More)
Preliminary incubation of platelets with prostaglandin E1(PGE1) led to the inhibition of changes in platelet shape caused by ADP. PGE1 also blocked changes in the content of membrane-bound calcium in platelets, which are known to take place under ADP-induced changes in platelet shape. Stable changes in platelet shape were observed on incubation in the(More)
The effects of trifluoperazine (TFP) on some membrane processes were studied in the isolated rat brain synaptic vesicles (SV). TFP (10(-5)-10(-4) M) was found to cause a sharp rise in the intensity of light scattering by SV suspension which was due both to an increased vesicle aggregation and to changes in the refraction index of the membrane. In addition,(More)
The properties of the transmembrane potential (TMP) of synaptosomes (Sin) from the rat brain cortex were studied by means of the fluorescent probe I-anilinonaphthalene-8-sulfonate (ANS). The fluorescent emission that increased at 464 nm (lambda ex = 365 nm) after addition of valinomycin to synaptosomes was considered as the reflection of Sin(More)
Transmembrane potential (TMP) of synaptic vesicles (SV) from the rat brain was registered by means of potential-sensitive fluorescent probe 3,3' dipropylthiocarbocyanine iodide (dis-C3-(5)). It was found that Mg-ATP generates TMP on the SV membrane suspended in sucrose-buffer medium. TMP did not appear if 0.25 M sucrose medium was replaced by 0.125 M KCl(More)
The applicability of the potential-sensitive dye diS-C3-(5) for the study of A23187 + Ca2+ induced plasma membrane hyperpolarization was tested in rat brain synaptosomes. An appropriate dye synaptosome ratio was chosen for the fluorescence titration dye in Ca-free Krebs-Ringer solution. The fluorescence intensity of the probe was increased upon the addition(More)
The effects of Mg-ATP, EGTA, EDTA and dicyclohexylcarbodiimide on the changes in the intensity of light scattering were studied in rat brain synaptic vesicles (SV) suspended in saccharose-buffer medium. Specific interactions between SV and isolated synaptic junctional complex were observed in the presence of Mg-ATP and calmodulin. An in vitro model of(More)
Preincubation of platelet suspension with dbcAMP during 2 minutes inhibited the ADP-induced change in platelet shape. An increase of the cAMP content in the cell induced by papaverine inhibiting phosphodiesterase also blocked the change in the shape caused by ADP and prostaglandin E2 (PGE2). Incubation of platelets in medium with pH 9.0 or addition of PGE2(More)
Mg-ATPase (1 mM) induces a decrease in the intensity of light scattering (I1) at 620 nm of rat brain synaptic vesicles (SV) suspended in sucrose, with this decrease being indicative of the swelling of the vesicles. The Mg-ATPase-induced swelling appears to be associated with the function of H+-ATPase of SV membranes, since it is completely abolished by the(More)
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