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Professional phagocytes such as neutrophils and macrophages can engulf invading microbes into phagosomes and then produce antimicrobial oxidants and enzymes in the phagosome to destroy the ingested microbes. Central to the oxidant-producing capability of a phagocyte is a transmembrane, multicomponent protein complex, a reduced nicotinamide adenine(More)
The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of(More)
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