I Ia Volozhantseva

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The biosynthesis of recombinant human lymphotoxin produced by E. coli SG20050/pLT21 cells and deprived of 21 amino acid residues has been studied. It has been shown that the bulk of the recombinant protein in E. coli cells is in the soluble form and predominantly localized in the cytoplasm. The maximal synthesis of soluble recombinant lymphotoxin is(More)
The involvement of the transposable DNA element of E. coli K12 chromosome in integrative recombination of RP1 plasmid was studied. Using temperature sensitive for replication plasmid RP1ts12--the derivative of RP1 which contains mutated transposon Tnl, it was shown that integration of RP1 into host chromosome and Hfr formation may occur according to a(More)
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