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The influence of semicarbazide (SC), an inhibitor of glutamate decarboxylase (GDC), on electrical and respiratory activity of the Purkinje cells and cortical neurons of mice was investigated in tissue culture. A biphase character of the SC influence on the investigated parameters was demonstrated. The first excitatory phase could be produced by the blocking(More)
Seasonal and circadian rhythms of neuronal and organism resistance to convulsive effect of strychnine and penicillin were studied in vivo on mice and rats and in vitro on cultured mouse cerebellar sections. Resistance was assessed by the latency of seizures in mice and neuronal response to convulsants in sections. In the night and morning time (0:00-9:00)(More)
Using survival slices of the rat cerebellum, we studied the influence of opiates (alpha- and beta-endorphines, met-enkephalines) as well as substance P (SP) on the impulse activity (IA) of neurons. Low doses of the studied substances (10(-8)-10(-10) M) for the most part increased the IA of the neurons, while high doses (10(6)-10(-5) M) produced biphasic(More)
Substance P in low concentrations (10(-7)M) activates rat cerebellar neurons (in slices), while in high concentrations (10(-6)and 10(-5)M) this compound causes a biphasic response (excitation-inhibition). Substance P probably acts as the excitatory neurotransmitter in the cerebellum and produces modulatory effects (triggering and facilitation) on cerebellar(More)
Spontaneous electrical activity, adenyl nucleotides' and cytochromes' contents, activity of respiratory chain enzymes complex in brain slices, homogenate and isolated mitochondria of rats, preliminary divided into non-resistant (NR) and high resistant (HR) animals by their sensitivity to hypobaric hypoxia have been investigated. It has been shown that in HR(More)
The direct effect of infralow-frequency (0.05, 0.1, 0.25 Hz, 100 nT) magnetic fields (MF) was demonstrated on the brain cellular--tissue model--surviving slices of mouse cerebellum. MF influence is a trigger for the nervous cells. MF-5 Hz revealed two-phases response: inhibition and excitation of the impulse activity of neurons. Besides that we recorded(More)
The effects of some antihypoxants (piracetam, GABA, sodium hydroxybutyrate and gutimine) on the electrical activity of the cerebellar neurons (Purkinje cells) of rats and mice were studied in the surviving slices under normoxia and increasing hypoxia. All the agents were found to produce phase changes in the base-line electrical activity of neurons:(More)
The functional activity of the neurons directly depends on the energy metabolism of the cell, and as the hypoxic effect grows there takes place an interreplaceability of metabolic flows supplying the respiratory chain with energy substrates and reducing equivalents participating in the compensatory maintenance of the energy status of the cell (glycolysis,(More)
Decrease of the pO2 led to the same phasic changes in the unit activity of Purkinje cells and hippocampal neurons in mice. Purkinje cells proved to be more resistant against oxygen deficiency in tissue culture than in surviving slices. Cells of the optic cortex preserved high level of functional activity at lowering the pO2 to 50%. Still, they were more(More)
The process of differentiation of neurons was traced on an organotypic cerebellar culture of newborn mice. Cerebellar cells reach morphological maturity by the 18-21st day of culturing. An increase of the membrane potential begins on the 6-7th day of culturing. It reaches values characteristic for the definitive stage of the neuron (65-75 mV) by the 9-10th(More)