I G Charlton

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OBJECTIVE To apply DNA image cytometry (ICM) to 53 tissue sections of breast cancer and skin melanomas. STUDY DESIGN DNA ICM was performed on 6-microns-thick sections with a commercially available ICM system, strictly following a previously developed measurement protocol. The histograms were classified according to Auer. RESULTS In 48 of the 53 cases(More)
Seventeen neuroendocrine neoplasms of the larynx were examined for DNA ploidy. Twelve tumours were atypical carcinoids, four paragangliomas and one a small cell neuroendocrine carcinoma. Seven atypical carcinoids were aneuploid, one diploid and one tetraploid, with metastasis being aneuploid. In three cases only skin metastases were available for study,(More)
Evaluation of the malignant potential of pancreatic endocrine tumours is difficult by histological criteria alone. Nuclear deoxyribonucleic acid (DNA) cytometric analysis can provide significant prognostic and biological information in a number of solid human tumours. Thus, the DNA profiles and nuclear parameters of 39 patients with pancreatic endocrine(More)
Most spontaneous first trimester abortions have an abnormal karyotype, some being triploid; the latter are said to show characteristic histological changes, including focal villous hydropic change, focal trophoblast hyperplasia, cisternal cavitation of larger villi, irregular outline to villi, and trophoblast inclusions. To date, DNA-ploidy profiles have(More)
We describe a modified method for examination of the atrioventricular and sinoatrial nodes that displays the major elements of the cardiac conducting system, yet is not time-consuming for the technician. We examined 31 cases in three categories: cases with previous cardiac history, sudden deaths from no known cause, and cases where death was sudden and(More)
OBJECTIVE To analyze the influence of measuring parameters on the quality of DNA histograms, and to compare the results of DNA image cytometry (ICM) and flow cytometry (FCM). STUDY DESIGN Material from 20 breast cancer cases was analyzed. The protocol for ICM analyzed (1) measuring system and image analysis parameters, (2) the quality of fixation, and (3)(More)
OBJECTIVE To determine whether combined quantitative immunohistochemistry of S-100, nuclear morphometry and DNA image cytometry improves discrimination between benign and malignant melanocytic skin lesions (MSLs). STUDY DESIGN S-100 protein expression was measured in tissue sections of MSLs using an image cytometry system. Localized areas of high S-100(More)
The ploidy profiles of benign and malignant tumours can be obtained using image analysis. However, the results of ploidy studies have varied according to the type of specimen used. We compared the ploidy profiles of paraffin embedded thin sections, cytospin preparations of disaggregated cells, and cytological smears from the same specimen as defined by(More)
OBJECTIVE To evaluate DNA image cytometry (ICM) in tissue sections, human and rat liver was used as a model. These tissues have distinct DNA ploidy histograms of mainly diploid (human) and predominantly tetraploid (rat) plus octaploid nuclei. STUDY DESIGN Section thickness varied from 4 to 12 microns in rat liver and 4 to 10 microns in human liver, in(More)
OBJECTIVE To examine the diagnostic value of DNA ploidy and nuclear morphometric features in sporadic dysplastic nevi as compared to those in compound nevi and melanoma. STUDY DESIGN DNA ploidy profiles plus seven direct and three derived nuclear features were obtained in a series of 120 melanocytic skin neoplasms (30 dysplastic nevi [DN], 30 melanomas(More)