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The pituitary glands were removed from 63 human fetuses from 5 weeks of gestation to term and studied by electron microscopy and ultrastructural immunocytochemistry to document the development of cell differentiation and hormone production in the adenohypophysis. At 5 weeks of gestation, Rathke's cleft was lined by columnar epithelium with abundant(More)
One hundred and forty human fetal pituitary glands were removed from fetuses at 7-40 weeks of gestation and studied by light microscopy and immunocytochemistry to localize adenohypophysial hormones. For immunocytology, the avidin-biotin-peroxidase complex technique was more sensitive and identified hormones in younger fetuses than did the immunoperoxidase(More)
Following 2 weeks of administration of 2-bromo-α-ergocryptine, a marked decrease was observed in prolactin immunoreactivity of the grafted pituitaries, whereas no reduction was noted in the intrasellar pituitaries. No evidence of crinophagy was revealed by electron microscopy in prolactin cells of 2-bromo-α-ergocryptine-treated rats.
With the use of electron microscopic morphology and immunochemistry, 5 tumors were studied: a spontaneous prolactin-producing adenoma (LEP rats); an estrogen-induced intrasellar tumor (R-Amsterdam rats); and 3 transplanted tumors, MtT.W10 and MtT.W5 (WF rats) and MtT.F4 (F344 rats). All tumors were derived from rat adenohypophysis and are known to secrete(More)
Electrolytic destruction of the pituitary stalk in rats markedly affected their water metabolism and induced ultrastructural changes in the posterior pituitary. The urinary output increased for 1-2 days after surgery. Subsequently polyuria and polydipsia temporarily decreased and vasopressin excretion with the urine increased. This oliguric interphase was(More)
Intrasellar and ectopic lactotrophs and somatotrophs of intact and hypophysectomized rats were studied by histology, immunohistochemistry, electron microscopy and ultrastructural morphometry after 2 weeks of TRH treatment. Both ectopic lactotrophs and somatotrophs of hypophysectomized rats showed subcellular changes indicative of stimulation following TRH(More)
Adenohypophyses of adult male rats have been investigated by light microscopy, immunocytology and electron microscopy 30 minutes, 1, 2, 4, 6 and 24 hours following electrolyte destruction of the pituitary stalk. Light microscopy revealed massive ischemic infarction of the adenohypophysis. Immunoreactive growth hormone, prolactin, TSH, FSH and LH were(More)
Intrasellar and transplanted pituitaries removed from the same rats were studied using light microscopy, immunocytology, electron microscopy and ultrastructural morphometry following treatment with bromocryptine and/or estrone acetate. Ectopic lactotrophs of rats treated with bromocryptine showed an increase in storage granule size and number, which was not(More)