I. A. Graskova

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The effects of the ring rot causal agent Clavibacter michiganensis subsp. sepedonicus (a virulent strain 5369) on the peroxidase activity of various tissues of potato plants grown under axenic conditions were studied. Root infection enhanced peroxidase activity in all plant tissues (roots, leaves, and stems). In the resistant cv. Lugovskoi, peroxidase(More)
89 Anaerobic non sporulating bacteria of the genus Clavibacter, representing single or paired (or short chains of) non motile rods, are the most widespread and significant among phytopathogen Gram positive bacteria. Clavibacter michiganensis subsp. sepedonicus (Spieck. et Kotth.) Skapt et Burkh (C. michiganensis) causes potato ring rot; as the result of(More)
Test-tube plants and suspension cell cultures of two cultivars of the potato (Solanum tuberosum L.) differing in their resistance to ring rot caused by Clavibacter michiganensis subsp. sepedonicus and six strains of this bacterium were used to test the relationship between the virulence, the leaf ability to adsorb bacteria, and the symptoms of the disease.(More)
425 In modern biology, the most important problem is an investigation of plant immunity mechanisms, in particular, toward various pathogens. Decease development and its consequences depend on the host-plant state and the activity of its defensive responses. Among other enzymes, peroxidases play an important role in plant defensive responses to pathogen(More)
Bacterial infection causes changes in plant metabolism that affect many enzymes, in particular, peroxidase, whose activity and spectrum are altered [1–4]. It is known that peroxidase in plants is represented by several isoforms differing in molecular weight and electrophoretic mobility [5, 6]. It was shown earlier that the infection of plants with the(More)
Various abiotic and biotic stressors cause significant changes in plant cell metabolism [1–3]. These changes are usually associated with triggering various signal systems that perceive the information on the stress and ultimately change gene expression [4]. Colonization of plants by pathogens is a strong stressor. It is known that plant infection triggers(More)
Infection of plants with a pathogen causes significant changes in plant metabolism. A characteristic response of plant cell to infection is the so-called oxidative burst, i.e., the generation of reactive oxygen species (ROS; H 2 O 2 , superoxide radicals, etc.). Oxidative burst represents a rapid transient production of ROS, which mat reach maximum in 15(More)
332 The investigation of plant metabolism during pathogenesis is important for understanding the mechanisms of plant damage by the pathogen and plant resistance to infection. It is known that metabolism of the plant cell experiences various disturbances during pathogenesis [1]. Cell membranes and proteins, enzymes in particular, are subjected to(More)
Changes in the activity of peroxidase, a component of the NADPH oxidase signaling pathway, in potato cells were studied. This activity increased sharply during ring rot pathogenesis. Two mechanisms of peroxidase activation were distinguished. One of them was the enzyme de novo synthesis; it was characteristic of the potato cultivar susceptible to the(More)
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