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Apple Flavonoid Phloretin Inhibits Escherichia coli O157:H7 Biofilm Formation and Ameliorates Colon Inflammation in Rats
  • Jin-Hyung Lee, S. Regmi, +4 authors Jintae Lee
  • Medicine, Biology
  • Infection and Immunity
  • 19 September 2011
Results suggest that the antioxidant phloretin also acts as an inhibitor of E. coli O157:H7 biofilm formation as well as an anti-inflammatory agent in inflammatory bowel diseases without harming beneficial commensal E. Escherichia coli biofilms. Expand
Cloning and Characterization of a Novel β-Transaminase from Mesorhizobium sp. Strain LUK: a New Biocatalyst for the Synthesis of Enantiomerically Pure β-Amino Acids
The enantioselectivity of the enzyme suggested that the recognition mode of β-aminocarboxylic acids in the active site is reversed relative to that of α-amino acids, which led to the deduced amino acid sequence of the β-transaminase. Expand
Revisit of aminotransferase in the genomic era and its application to biocatalysis
Abstract Aminotransferases (ATs) have useful applications in the chemical industry because of their capability of introducing amino group into ketones or keto acids as well as their highExpand
Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system
Phenolic acid decarboxylase (PAD) catalyzes the non-oxidative decar boxylation of p-coumaric acid (pCA) to p-hydroxystyrene (pHS) and was found as the most active one using the “Subgrouping Automata” program and by comparing enzyme activity. Expand
Kinetic resolution of (R,S)‐sec‐butylamine using omega‐transaminase from Vibrio fluvialis JS17 under reduced pressure
The kinetic resolution of (R,S) sec‐butylamine with the ω‐transaminase (TA) from Vibrio fluvialis JS17 was performed under reduced pressure (e.g., 150 torr) to selectively remove an inhibitoryExpand
Stereospecific Synthesis of (R)‐2‐Hydroxy Carboxylic Acids Using Recombinant E. coli BL21 Overexpressing YiaE from Escherichia coli K12 and Glucose Dehydrogenase from Bacillus subtilis
The yiaE gene from Escherichia coli K12 was functionally expressed in E. coliBL21 using an IPTG inducible pET expression system, and YiaE was purified to a specific activity of 18 U/mg, and the problem of NADPH recycle was effectively solved by using recombinantE. Expand
Production of ω-hydroxy palmitic acid using CYP153A35 and comparison of cytochrome P450 electron transfer system in vivo
Various CamAB expression systems according to gene arrangements of the three proteins and promoter strength in their gene expression were compared in terms of product yields and productivities. Expand
omega-Amino acid:pyruvate transaminase from Alcaligenes denitrificans Y2k-2: a new catalyst for kinetic resolution of beta-amino acids and amines.
Alcaligenes denitrificans Y2k-2 was obtained by selective enrichment followed by screening from soil samples, which showed omega-amino acid:pyruvate transaminase activity, to kinetically resolveExpand
ω-Transaminases for the Production of Optically Pure Amines and Unnatural Amino Acids
ω-Transaminases have been increasingly used as efficient biocatalysts due to their ability to produce a wide range of optically pure amine compounds. Several approaches have been adopted, includingExpand