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Extracellular recombinant protein production from an Escherichia coli lpp deletion mutant
This study addresses the outer membrane barrier for the translocation of recombinant protein directed to the periplasmic space by using recombinant maltose binding protein, xylanase, and cellulase as model proteins, and investigates whether the lpp deletion could render the outer membranes permeable enough to allow extracellular protein production. Expand
Production and characterization of a type B feruloyl esterase from Fusarium proliferatum NRRL 26517
A novel feruloyl esterase was discovered from a Fusarium proliferatum strain known to be capable of utilizing corn fiber xylan, and its unusual insensitivity to pH makes it a potentially useful enzyme in applications that require acidic or alkaline conditions. Expand
Comparative genomics and transcriptome analysis of Aspergillus niger and metabolic engineering for citrate production
Comparative genomics and transcriptome analysis of citrate-producing strains revealed that the transcription levels of 479 genes changed between the cell growth stage and the citrate synthesis stage, and two cytosol ATP-citrate lyases were up-regulated and may coordinate with the alternative oxidases in the alternative respiratory pathway for energy balance. Expand
Soil treatment using microbial biopolymers for anti-desertification purposes
Abstract Desertification and soil degradation are becoming more serious due to global warming and concurrent extreme climate events. Although anti-desertification efforts have been mounted worldwide,Expand
A complete enzymatic recovery of ferulic acid from corn residues with extracellular enzymes from Neosartorya spinosa NRRL185
The discovery of a filamentous fungus Neosartorya spinosa NRRL185 capable of producing a full complement of enzymes to release ferulic Acid and the development of an enzymatic process for a complete recovery of ferulic acid from corn bran and corn fibers are reported. Expand
Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology
The available genetic tools, engineering strategies, genome sequence, genome-scale structure models, proteome, and secretion pathways, and successful examples of enzymes and industrially important biochemicals produced by Bacillus spp. Expand
Escherichia coli Binary Culture Engineered for Direct Fermentation of Hemicellulose to a Biofuel
This is the first demonstration of an engineered binary culture for consolidated bioprocessing of xylan, a complex substrate requiring six hemicellulases for complete hydrolysis, and the modular design should allow the strategy to be adopted for a broad range of biofuel and biorefinery products. Expand
A recombinant E. coli bioprocess for hyaluronan synthesis
A potentially viable process for HA based on a recombinant E. coli strain engineered into an efficient hyaluronic acid producer is demonstrated and the precursor supply limitation identified in this study suggests new engineering targets in subsequent metabolic engineering efforts. Expand
Systems-level understanding of how Propionibacterium acidipropionici respond to propionic acid stress at the microenvironment levels: mechanism and application.
It was indicated that the acid tolerance of P. acidipropionici was systematically regulated, and the increased arginine, aspartic acid, and glutamic acid concentrations helped to resist the acidic environment by consuming more H⁺ and generating more ATP and NH₃. Expand
Amplification of the NADPH-related genes zwf and gnd for the oddball biosynthesis of PHB in an E. coli transformant harboring a cloned phbCAB operon.
The amount ofPHB increased after enforcing the genes; especially the zwf gene an increase of around 41%, due to the rise in NADPH and the depressed TCA cycle, leading to the metabolic flux of intermediates to the pathway for the biosynthesis of PHB. Expand