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Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective(More)
In animals such as the fruitfly, even minor deviations in environmental temperature can have major impacts on development and lifespan. Here we demonstrated that the ability of Drosophila melanogaster larvae to discriminate between the optimal temperature of 18 degrees C and slightly higher temperatures (19-24 degrees C) depended on the TRPA1 channel, which(More)
Animals select their optimal environmental temperature, even when faced with alternatives that differ only slightly. This behavior is critical as small differences in temperature of only several degrees can have a profound effect on the survival and rate of development of poikilothermic animals, such as the fruit fly. Here, we demonstrate that Drosophila(More)
Here, we show that a subset of breast cancers express high levels of the type 2 phosphatidylinositol-5-phosphate 4-kinases α and/or β (PI5P4Kα and β) and provide evidence that these kinases are essential for growth in the absence of p53. Knocking down PI5P4Kα and β in a breast cancer cell line bearing an amplification of the gene encoding PI5P4K β and(More)
  • Bryce Daines, Hui Wang, Yumei Li, Yi Han, Richard Gibbs, Rui Chen +78 others
  • 2009
Using RNA interference to identify specific modifiers of a temperature-sensitive, embryonic-lethal mutation in the Caenorhabditis elegans ubiquitin-like Nedd8 protein modification pathway E1-activating gene rfl-A screen for modifiers of notch signaling uncovers Amun, a protein with a critical role in sensory organ development .. Analysis of Pax6 contiguous(More)
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