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This paper illustrates the benejits, for a wide range of design activities, of Interactive Visualization Artifacts. INTRODUCTION In an extremely wide range of design tasks, of which electronic product design is an example, the designer is concerned with the influence of the parameters whose values can be chosen upon the performances which are of direct(More)
In this paper we present two novel visualisation tools: the Influence Explorer and the Prosection Matrix. These were specifically created to support engineering artifact design and similar tasks in which a set of parameter values must be chosen to lead to acceptable artifact performance. These tools combine two concepts. One is the interactive and virtually(More)
Copper amine oxidases have a complex reaction cycle that converts a primary amine and molecular oxygen into the aldehyde, ammonia and hydrogen peroxide. Coupling structural studies of freeze-trapped reaction intermediates in crystals with kinetic and spectroscopic experiments in solution has generated a detailed molecular picture of catalysis. Although(More)
Analytical ultracentrifugation (AUC) has re-emerged as a powerful technique for protein characterisation. We report the pivotal role sedimentation equilibrium AUC has played in the development of macrophage inflammatory protein-1α (MIP-1α) as a protein therapeutic. MIP-1α has potential clinical applications in cancer but its clinical use is limited, since(More)
Galactose oxidase (GO; E.C. 1.1.3.9) is a copper- containing enzyme that oxidizes a range of primary alcohols to aldehydes. This broad substrate specificity is reflected in a high K(M) for substrates. Directed evolution has previously been used to select variants of GO that exhibit enhanced expression and kinetic properties. In assays using unpurified(More)
Galactose oxidase (GO; E.C. 1.1.3.9) is a coppercontaining enzyme that oxidizes a range of primary alcohols to aldehydes. This broad substrate speci®city is re ̄ected in a high KM for substrates. Directed evolution has previously been used to select variants of GO that exhibit enhanced expression and kinetic properties. In assays using unpuri®ed enzyme(More)
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