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To study the Ca2+/Calmodulin (CaM) signal transduction pathway of Gracilaria lemaneiformis under heat stress, myo-inositol-1-phosphate synthase (MIPS), a calmodulin-binding protein, was isolated using the yeast two-hybrid system. cDNA and DNA sequences of mips were cloned from G. lemaneiformis by using 5′RACE and genome walking procedures. The MIPS DNA(More)
Clustered regularly interspaced short palindromic repeats (CRISPR), which are widely present in prokaryotic genomes (Grissa et al., 2007), belong to a family of DNA sequences characterized as short direct repeats (DR) separated by spacers (Jansen et al., 2002). CRISPR and CRISPR-associated (cas) genes are involved in resistance against exogenous sequences,(More)
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