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Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L.
Modern sugarcanes are polyploid interspecific hybrids, combining high sugar content from Saccharum officinarum with hardiness, disease resistance and ratooning of Saccharum spontaneum. Sequencing of
Methanocaldococcus jannaschii Uses a Modified Mevalonate Pathway for Biosynthesis of Isopentenyl Diphosphate
TLDR
Unlike other known kinases associated with isoprene biosynthesis, Methanocaldococcus jannaschii isopentenyl phosphate kinase is predicted to be a member of the aspartokinase superfamily.
( R )-Citramalate Synthase in Methanogenic Archaea
TLDR
The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate.
Identification of an Archaeal 2-Hydroxy Acid Dehydrogenase Catalyzing Reactions Involved in Coenzyme Biosynthesis in Methanoarchaea
TLDR
It is concluded that the MJ1425-encoded enzyme is likely to be involved in the biosynthesis of both coenzyme M and methanopterin.
Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis.
TLDR
The MJ0887 gene product has been designated CofC to indicate its involvement in the third step of coenzyme F 420 biosynthesis.
Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F420 biosynthesis
TLDR
A molecular phylogeny of the paralogous cofG and cofH genes is consistent with the genes being vertically inherited within the euryarchaeal, cyanobacterial, and actinomycetal lineages.
Identification of Coenzyme M Biosynthetic Phosphosulfolactate Synthase
TLDR
An enzyme from that organism, (2R)-phospho-3-sulfolactate synthase (ComA), that catalyzes the first step in coenzyme M biosynthesis, and its Mg2+-dependent enzyme reaction mechanism may be analogous to one proposed for enolase.
Conformational Fluctuations Coupled to the Thiol-Disulfide Transfer between Thioredoxin and Arsenate Reductase in Bacillus subtilis*
TLDR
A comparison of the complex structure with that of thioredoxin and arsenate reductase proteins in redox states showed substantial conformational changes coupled to the reaction process, with arsenate Redux adopting an “intermediate” conformation in the complex.
Glutathione synthetase homologs encode α-l-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses
TLDR
Two members of this family have been identified in the hyperthermophilic marine archaeon Methanococcus jannaschii and shown to catalyze the final reactions in two coenzyme biosynthetic pathways, illustrating convergent evolution of nonribosomal peptide biosynthesis through the recruitment of single-step amino acid ligases.
A member of a new class of GTP cyclohydrolases produces formylaminopyrimidine nucleotide monophosphates.
TLDR
The hyperthermophilic euryarchaeon Methanococcus jannaschii has no recognizable homologues of the canonical GTP cyclohydrolase enzymes that are required for riboflavin and pteridine biosyntheses and uses a new type of thermostable GTP cycling enzyme that produces 2-amino-5-formylamino-6-ribonucleotide monophosphate and inorganic phosphate.
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