Hui-fang Yan

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Identification of efficient key enzymes in biosynthesis pathway and optimization of the fitness between functional modules and chassis are important for improving the production of target compounds. In this study, the taxadiene biosynthesis pathway was firstly constructed in yeast by transforming ts gene and overexpressing erg20 and thmgr. Then, the(More)
OBJECTIVE To evaluate the chromium (Cr) levels in blood and urine among general population in China between 2009 and 2010, and thereby to analyze its prevalent features. METHODS From year 2009 to 2010, a total of 11 983 subjects of general population aged between 6 and 60 year-old were recruited from 24 districts in 8 provinces in eastern, central and(More)
OBJECTIVE To establish an inductively coupled plasma mass spectrometry(ICP-MS) method for determination of 30 trace elements including As, Ba, Be, Bi, Ni, Cd, Co, Cr, Cs, Cu, Ga, Mn, Pb, Sr, Tl, V, Ge, Mo, Nb, Ti, W, Te, Se, Zr, In, Sb, Hg, Ce, La, and Sm in human blood. METHOD The blood samples were analyzed by ICP-MS after diluted 1/10 with 0.01%(More)
OBJECTIVE A sampling method was established to collect diborane in the air of workplace and an ICP-AES method was developed to determine the Boron in desorbed solution. METHOD Diborane in the air of workplace was collected by solid sorbent tube filled with oxidant impregnated activated carbon. The adsorbed diborane was desorbed into 3% H2O2 aqueous, and(More)
OBJECTIVE An atomic fluorescence (AFS) method was developed to determine germanium hydride in the air of workplace. METHOD Germanium hydride in the air of workplace was collected by charcoal tube, and desorbed by nitric acid followed filtration with 0.22 microm cellulose filter, the AFS was used to determine Germanium in the desorbed solution. RESULTS(More)