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T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface.
TLDR
It is suggested that the interplay between multiple Tim-3-binding activities contributes to the regulated assembly of signaling complexes required for effective Th1-mediated immunity. Expand
A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.
TLDR
ML323 is reported, a highly potent inhibitor of the USP1-UAF1 deubiquitinase complex with excellent selectivity against human DUBs, deSUMOylase, deneddylase and unrelated proteases, pointing to USP 1- UAF1 as a key regulator of the DNA damage response and a target for overcoming resistance to the platinum-based anticancer drugs. Expand
Insights into the mechanism of microtubule stabilization by Taxol
TLDR
It is demonstrated that HDX coupled to liquid chromatography–electrospray ionization MS can be effectively used to study conformational effects induced by small ligands on microtubules, and opens avenues for locating drug and protein binding sites and for deciphering the mechanisms by which their interactions alter the conformation of microtubule and tubulin dimers. Expand
Hallmarks of Molecular Action of Microtubule Stabilizing Agents
TLDR
A comprehensive analysis of the effects of epothilone B, ixabepilone, laulimalide, and peloruside A on CET conformation, indicating that all MSAs have significant conformational effects on the C-terminal H12 helix of α-tubulin, which is a likely molecular mechanism for the previously observed modulations of microtubule-associated and motor proteins. Expand
Cytosolic Carboxypeptidase 1 Is Involved in Processing α- and β-Tubulin*
TLDR
Results demonstrate a role for CCP1 in the processing of Glu residues from β- as well as α-tubulin in vitro and in vivo, and counteracted by the knock-out of the gene encoding tubulin tyrosine ligase-like-1, indicating that this enzyme hyperglutamylates α- and β- Tubulin. Expand
Mapping protein energy landscapes with amide hydrogen exchange and mass spectrometry: I. A generalized model for a two‐state protein and comparison with experiment
TLDR
The goal of this work is to develop a framework for a generalized exchange model that takes into account multiple processes leading to amide hydrogen exchange, and does not require that the exchange proceed strictly via EX1 or EX2 kinetics. Expand
Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states
TLDR
Data indicate that PAmCherry1 in the dark state possesses the chromophore N-[(E)-(5-hydroxy-1H-imidazol-2-yl)methylidene]acetamide, which, to the authors' knowledge, has not been previously observed in PAFPs. Expand
Detection of human betaV-tubulin expression in epithelial cancer cell lines by tubulin proteomics.
TLDR
The data confirm that human and rodent betaV-tubulins are distinct and indicate that, regardless of species, betaIII- and beta V-tubulin may be expressed in a complementary pattern at the protein level. Expand
Termination of Protofilament Elongation by Eribulin Induces Lattice Defects that Promote Microtubule Catastrophes
TLDR
It is demonstrated that single Eribulin molecules specifically interact with microtubule plus ends and are sufficient to either trigger a catastrophe or induce slow and erratic microtubules growth in the presence of EB3. Expand
The Histone Code of Toxoplasma gondii Comprises Conserved and Unique Posttranslational Modifications
TLDR
The characterization of the T. gondii histone code will facilitate in-depth analysis of how epigenetic regulation affects gene expression in pathogenic apicomplexan parasites and identify a new model system for elucidating the biological functions of novel histone PTM. Expand
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