Hui-Min Chen

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The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system has emerged recently as a powerful method to manipulate the genomes of various organisms. Here, we report a toolbox for high-efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA)(More)
Host defense consists of two main aspects, namely, immune response to invading pathogens and suppression of tumor development. A family of transcription factors, IFN regulatory factors (IRFs), has recently gained much attention in terms of its critical role in linking these two aspects of host defense, wherein IRF5 was previously shown to play a critical(More)
Users move from one state (or task) to another in an information system’s labyrinth as they try to accomplish their work, and the amount of time they spend in each state varies. This article uses continuous-time stochastic models, mainly based on semi-Markov chains, to derive user state transition patterns (both in rates and in probabilities) in a Web-based(More)
Echinoid (Ed) is a homophilic immunoglobulin domain-containing cell adhesion molecule (CAM) that localizes to adherens junctions (AJs) and cooperates with Drosophila melanogaster epithelial (DE)-cadherin to mediate cell adhesion. Here we show that Ed takes part in many processes of dorsal closure, a morphogenetic movement driven by coordinated cell shape(More)
Aberrant methylation has been shown to trigger the inactivation of tumor suppressor genes during tumorigenesis. MicroRNAs (miRNAs) have been found deregulated in human colorectal cancer (CRC), and some of them may function as tumor suppressor genes. Here, we investigated CpG island promoter hypermethylation as a potential mechanism underlying miRNA(More)
BACKGROUND Accumulating evidence indicates that diet is one of the most important environmental factors involved in the progression from advanced colorectal adenoma (A-CRA) to colorectal cancer. OBJECTIVE We evaluated the possible effects of dietary fiber on the fecal microbiota of patients with A-CRA. DESIGN Patients with a diagnosis of A-CRA by(More)
Ends-out gene targeting allows seamless replacement of endogenous genes with engineered DNA fragments by homologous recombination, thus creating designer "genes" in the endogenous locus. Conventional gene targeting in Drosophila involves targeting with the preintegrated donor DNA in the larval primordial germ cells. Here we report G: ene targeting during O:(More)
To evaluate the relationship between age and clinicopathological characteristics in gastric carcinoma patients, we performed the meta-analysis based on nine retrospective clinical trials. Comparing elderly patients with young patients it showed lower male/female ratio, more diffuse GC, more Borrmann type IV, more poorly differentiated carcinoma, more(More)
Intergeneric somatic hybrids were obtained by fusion between protoplasts of Triticum aestivum L. cv. Jinan 177 and Leymus chinensis (Trin.) Tzvel. Protoplasts of L. chinensis were exposed to UV (300 microW/cm(2)) for 30 s, 45 s and 1 min before fusion. The results of morphological and chromosomal observation, isozyme pattern as well as RAPD analysis and the(More)