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Clinical studies have shown that the tyrosine kinase inhibitor STI571 effectively controls BCR-ABL-positive chronic myelogenous leukemia (CML). However, disease progression while on STI571 therapy has been reported, suggesting de novo or intrinsic resistance to BCR-ABL-targeted therapy. To investigate possible mediators of acquired STI571 resistance, K562(More)
Imatinib mesylate (IM) binds to the BCR-ABL protein, inhibiting its kinase activity and effectively controlling diseases driven by this kinase. IM resistance has been associated with kinase mutations or increased BCR-ABL expression. However, disease progression may be mediated by other mechanisms that render tumor cells independent of BCR-ABL. To(More)
The budC gene coding for a new meso-2,3-butanediol dehydrogenase (BDH) from Serratia marcescens H30 was cloned and expressed in Escherichia coli BL21(DE3), purified, and characterized for its properties. The recombinant BDH with a molecular weight of 27.4 kDa exhibited a reversible transformation between acetoin and 2,3-butanediol. In the presence of NADH,(More)
To take full advantage of locked nucleic acid (LNA) based molecular beacons (LNA-MBs) for a variety of applications including analysis of complex samples and intracellular monitoring, we have systematically synthesized a series of DNA/LNA chimeric MBs and studied the effect of DNA/LNA ratio in MBs on their thermodynamics, hybridization kinetics, protein(More)
—In this letter, a new audio fingerprinting approach is presented. We investigate to improve robustness by more precise statistical fingerprint modeling with common component Gaussian mixture models (CCGMMs) and Kullback–Leibler (KL) distance, which is more suitable to measure the dissimilarity between two probabilistic models. To address the resulting(More)
The strain Achromobacter sp. JA81, which produced enoate reductase, was applied in the asymmetric reduction of activated alkenes. The strain could catalyze the bioreduction of alkenes to form enantiopure (R)-β-aryl-β-cyano-propanoic acids, a precursor of (R)-γ-amino butyric acids, including the pharmaceutically active enantiomer of the chiral drug(More)
Serratia sp. T241, a newly isolated xylose-utilizing strain, produced three 2,3-butanediol (2,3-BD) stereoisomers. In this study, three 2,3-butanediol dehydrogenases (BDH1-3) and one glycerol dehydrogenase (GDH) involved in 2,3-BD isomers formation by Serratia sp. T241 were identified. In vitro conversion showed BDH1 and BDH2 could catalyzed (3S)-acetoin(More)
— It is observed that the cepstral-based features used for speech recognition are sensitive to some auxiliary information (e.g. pitch). Encoding the auxiliary information in discrete auxiliary variables based on dynamic Bayesian networks (DBNs) typically results in an increased number of parameters. There are tradeoffs to be studied between parameter(More)
This paper investigates the problem of incorporating auxiliary information (e.g. pitch) for speech recognition using dynamic Bayesian networks (DBNs). Previous works usually model acoustic features conditional on the pitch auxiliary variable for both voiced and unvoiced phonetic states, and therefore ignore the fact that pitch (frequency) information is(More)
Recent studies have indicated that the inflamma-tory microenvironment plays a significant role in colorectal cancer (CRC). The interleukin‑6/signal transducer and activator of transcription 3 (IL-6/STAT3) signaling pathway mediates the proliferative and anti‑apoptotic activities required for oncogenesis under inflammatory conditions; thus, suppressing tumor(More)