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Addition of acetate to a stationary phase culture of Escherichia coli in glycerol mineral salts medium containing phosphorus-32-labeled orthophosphate results in rapid loss of isocitrate dehydrogenase activity and concomitant incorporation of phosphorus-32 into the enzyme. This is the first example of protein phosphorylation in a bacterium in which the(More)
The intracellular localization of isocitrate dehydrogenase was determined by immunochemical techniques with ultrathin sections of Escherichia coli. The thin sections, which were obtained by ultracryomicrotomy, were incubated first with antiserum specific for the enzyme and then with a protein A-gold complex. Transmission electron microscopy showed that the(More)
ICD-Edit version 1.7.1 is a service for user interaction with 3D diagrams. It can act as a presentation-layer process or as a diagram editor | or as both combined. This document deenes the diagrams that it can display, languages for deening their graphical content and the protocol for use of this service by a client process. Also touched on are how this(More)
BACKGROUND Robotic-assisted laparoscopic prostatectomy (RALP) has greatly changed clinical management of prostate cancer. It is important for pathologists and urologists to compare RALP with conventional open radical retropubic prostatectomy (RRP), and evaluate their effects on surgical pathology specimens. METHODS We retrospectively reviewed and(More)
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