Hugh K. Ward

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A large proportion (30%) of tissue glutaminase activity was found localized in synaptosome fractions as well as purified mitochondrial fractions, where it is also enriched on a protein basis (2-fold). Sulphate was more effective (2-8-fold) than phosphate or chloride in activating the enzyme at concentrations above 10-12 mM, but equivalent to phosphate at(More)
Radiolabelled glutamine and glucose were infused into lateral ventricles of rats in order to label transmitter amino acid pools in vivo. Brain regions close to the lateral ventricle (hippocampus, corpus striatum, hypothalamus) were labelled more effectively than more distant structures such as cerebral cortex or cerebellum. All regions were labelled to much(More)
The activities of several enzymes involved in the metabolism of aspartate and glutamate were measured in striatal (nucleus caudatus and putamen) homogenates 2-3, 6-7, and 35-40 days following frontoparietal and frontal cortical ablation. The activity of glutamine synthetase (GS) was substantially increased (46-48%) on the operated side 6-7 days following(More)
Cerebrocortical synaptosomes were incubated with glutamine together with 6-diazo-5-oxo-L-norleucine (DON) and NH4Cl (which are known to inhibit phosphate-stimulated glutaminase) in order to assess the effect of such inhibition on the pool sizes and extent of evoked release of endogenous amino acids, particularly glutamate. DON (5 mM) inhibited glutaminase(More)
In anesthetized dogs the popliteal lymph node alone, and the popliteal and iliac lymph nodes in series, have been perfused with solutions containing dog erythrocytes and streptococci. The perfusions have been carried out under conditions of lymph flow and pressure within the limits of those occurring in the actively moving dog, or after a severe degree of(More)
The respiratory rate, potassium, amino acid, ATP and phosphocreatine content of cortical synaptosomes were measured over periods from one hour to 5 days. Incubation medium or tissue culture medium at 37 degrees C was employed. In suspensions, synaptosomes show diminished levels of all these parameters after 3 or 4 h, but synaptosome beds showed no loss of(More)
The rates of phosphate activated glutaminase activity in finely homogenised cerebral cortex and synaptosomes were measured. Activity was 25–50% higher at pH 7.0 than at pH 8.0. Glutamate inhibited activity with a Ki of 2–3 mM while aspartate had little effect. Calcium (1 mM) activated the enzyme but magnesium was without action. The pH profiles of the(More)
Pulmonary complications occur in an estimated 0.21% of patients with inflammatory bowel disease. The most common presentation of pulmonary manifestations is large airway disease, such as tracheobronchitis, chronic bronchitis or bronchiectasis. Small airway disease, such as constrictive bronchiolitis or bronchiolitis obliterans with organizing pneumonia, is(More)