Hubert Zipper

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The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time PCR. Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure-property relationships that affect methods applying SG. These studies(More)
Metagenome cloning has become a powerful tool to exploit the biocatalytic potential of microbial communities for the discovery of novel biocatalysts. In a novel variant of direct expression cloning, metagenomic DNA was isolated from compost by a modified direct lysis method, purified by size exclusion chromatography and cloned into an expression vector(More)
DNA quantification of soils and sediments is useful for the investigation of microbial communities and for the acquisition of their genomes that are exploited for the production of natural products. However, in such samples DNA quantification is impaired by humic acids (HA). Due to its lack of specificity and sensitivity, UV spectrophotometry cannot be(More)
Within the framework of food surveillance a total number of 593 conventionally grown strawberry samples mainly originating from Germany, Spain, Italy and Morocco were analysed for pesticide residues at the Chemisches und Veterinäruntersuchungsamt Stuttgart between 2002 and 2005 using the QuEChERS multiresidue method. Pesticide residues were detected in 98%(More)