Functional analysis of the ver gene using antisense transgenic wheat.
- K. Chong, Shilai Bao, Zhi-hong Xu
- Medicine, BiologyPhysiologia Plantarum : An International Journal…
- 1 January 1998
The function of ver203, a gene related to vernalization in winter wheat, was investigated by expression of a complementary DNA as an antisense RNA in transgenic plants, and results suggested that the VER203 protein plays an important role in controlling heading and flower development inWinter wheat.
Overexpression of DsbC and DsbG markedly improves soluble and functional expression of single-chain Fv antibodies in Escherichia coli.
- Zhong Zhang, Zhi-hua Li, Hua-liang Huang
- BiologyProtein Expression and Purification
- 1 November 2002
Production of soluble and functional engineered antibodies in Escherichia coli improved by FkpA.
- Zhong Zhang, Li-ping Song, Hua-liang Huang
- BiologyBioTechniques
- 1 November 2003
It is reported that fusion or co-expression of FkpA, the E. coli periplasmic peptidyl-prolyl-isomerase with chaperone activity, substantially improves soluble and functional expression of scAbs.
Targeting TNF-α with a tetravalent mini-antibody TNF-TeAb
- Mengyuan Liu, Xiang-bin Wang, Hua-liang Huang
- Biology
- 1 September 2007
It is shown that multimerization of the antibody fragment by a self-association peptide is an efficient way to increase its avidity and that TNF-TeAb has potential applicability for anti-TNF-α therapy.
Targeting TNF-alpha with a tetravalent mini-antibody TNF-TeAb.
- Mengyuan Liu, Xiang-bin Wang, Hua-liang Huang
- BiologyBiochemical Journal
- 2007
It is shown that multimerization of the antibody fragment by a self-association peptide is an efficient way to increase its avidity and that TNF-TeAb has potential applicability for anti-TNF-alpha therapy.
Construction of a fully synthetic human scFv antibody library with CDR3 regions randomized by a split-mix-split method and its application.
- Chang-cheng Yin, Lifen Ren, Xichong Yan
- BiologyJournal of Biochemistry (Tokyo)
- 1 November 2008
The results proved the feasibility of the split-mix-split DNA randomization strategy in library construction and site-directed mutagenesis and the utility was demonstrated by screening of scFv clones against BHL.
One‐step on‐column purification and refolding of a single‐chain variable fragment (scFv) antibody against tumour necrosis factor α
- Mengyuan Liu, Xiang-bin Wang, Hua-liang Huang
- BiologyBiotechnology and applied biochemistry
- 1 March 2006
Activity assays showed that refolded TNF‐scFv could bind to rhTNFα (recombinant human TNFα) specifically with high affinity and could inhibit rhT NFα from binding to TNF receptors and neutralize the cytolytic activity of rhTNIα against L929 cells effectively.
A new recombinant single chain trispecific antibody recruits T lymphocytes to kill CEA (carcinoma embryonic antigen) positive tumor cells in vitro efficiently.
- Xiang-bin Wang, Baofeng Zhao, Hua-liang Huang
- BiologyJournal of Biochemistry (Tokyo)
- 1 April 2004
A new single chain trispecific antibody (CEA x CD3 x CD28) antibody was constructed and characterized carefully and was found to possess functions to activate T lymphocytes independently of additional co-stimulatory signal, and to attract activated T lymphocyte around CEA-positive tumor cells, which resulted in effective cytolysis of tumor cells.
A novel bivalent single‐chain variable fragment (scFV) inhibits the action of tumour necrosis factor α
- Mengyuan Liu, Xiang-bin Wang, Hua-liang Huang
- BiologyBiotechnology and applied biochemistry
- 1 August 2008
A bivalent scFv (single‐chain variable fragment) fragment, named TNF‐BAb, was engineered by fusing two anti‐TNFα scFV fragments in tandem via a long and flexible linking peptide derived from human serum albumin and produced in functional form from Escherichia coli inclusion bodies to demonstrate the ability to inhibit the biological action of TNFα.
Molecular cloning and characterization of vernalization‐related (ver) genes in winter wheat
- K. Chong, Li-Ping Wang, K. Tan, Hua-liang Huang, Houguo Liang
- Biology
- 1 November 1994
Results of the hybridization in situ of differential screening with probes of the control, the vernalization and devernalized wheat cDNAs showed that verc 17 (verc: vernAlization-relatcd cDNA clone) is specific for the veinalization.
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