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A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal(More)
A sensitive and specific monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) was established and validated for the detection of staphylococcal enterotoxin A (SEA). After routine fusion and selection, 10 monoclonal antibodies showed high affinity for SEA. An optimal pair for sandwich ELISA was selected by pairwise interaction(More)
An ultrasensitive and rapid electrochemical platform for the specific detection of ochratoxin A (OTA) was developed. In this method, three single-stranded DNA molecules, including the aptamer, were immobilized on the surface of an electrode. Binding of the OTA target analyte to the aptamer changed the redox current of methylene blue (MB), which was used as(More)
In this paper, we describe the development of a multicomponent lateral-flow assay based on an antibody-antigen reaction for the rapid and simultaneous detection of trace contaminants in water, including a heavy metal, algal toxin, antibiotic, hormone, and pesticide. The representative analytes chosen for the study were lead (Pb(II),(More)
A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive monoclonal antibody (mAb) for CIP was generated by immunizing BALB/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP(More)
A monoclonal antibody for microcystin-leucine-arginine (MC-LR) was produced by cell fusion. The immunogen was synthesized in two steps. First, ovalbumin/ bovine serum albumin was conjugated with 6-acetylthiohexanoic acid using a carbodiimide EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride)/ NHS (N-hydroxysulfosuccinimide) reaction. After(More)
An extended optimal velocity traffic flow model on two lanes including a bus stop and a bus deceleration area is proposed. The fundamental diagram is classified into seven distinct traffic states with the variation of density. Two new traffic states are found. One of them shows that lane-changing occurs frequently whether in front of or behind the bus, the(More)
The fluorescence-encoded microspheres were prepared by the simple and controllable microfluidic device for the first time. The polycaprolactone (PCL) microspheres were encoded with different quantum dots (QDs) at various ratios, and the fluorescence was decoded successfully. The designed fluorescence encoding method was easily manipulated, and generated the(More)
Controllable assembly of nanoscale building blocks (monomers) is a necessary part of practical realization of the unique optical, electrical, magnetic, and chemical properties of nanoscale matter in macroscale materials. Such assemblies also contain much fundamental information about collective behavior of nanocolloids, which we are just beginning to(More)
Bisphenol A (BPA), a chemical widely used in the manufacture of polycarbonate plastics, has raised considerable concern in recent decades because of its hormone-like properties. Whether BPA exposure is a health risk remains controversial in many countries. A metabolomics study based on capillary electrophoresis time-of-flight mass spectrometry (CE-TOF/MS)(More)