Hsiao-Yun Huang

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tRNAs in yeast and vertebrate cells move bidirectionally and reversibly between the nucleus and the cytoplasm. We investigated roles of members of the beta-importin family in tRNA subcellular dynamics. Retrograde import of tRNA into the nucleus is dependent, directly or indirectly, upon Mtr10. tRNA nuclear export utilizes at least two members of the(More)
Statistical discrimination for nonstationary random processes is important in many applications. Our goal was to develop a discriminant scheme that can extract local features of the time series, is consistent, and is computationally efficient. Here, we propose a discriminant scheme based on the SLEX (smooth localized complex exponential) library. The SLEX(More)
Class imbalance problem has attracted many attentions in recent years. When the available training sample size of each class is imbalanced, the directly established classification model will tend to allocate the testing sample into the majority class. A proper resampling method together with a power classifier is generally employed for dealing with this(More)
tRNAs perform an essential role in translating the genetic code. They are long-lived RNAs that are generated via numerous posttranscriptional steps. Eukaryotic cells have evolved numerous layers of quality control mechanisms to ensure that the tRNAs are appropriately structured, processed, and modified. We describe the known tRNA quality control processes(More)
Ubiquitous computing is aiming at providing users with intelligent human-centric context-aware services at anytime anywhere. However, mobility increases dynamism and uncertainty conditions. This study therefore explores the management and uses of various contexts for automatically providing appropriate services to individual users. This issue is explored(More)
Bidirectional tRNA movement between the nucleus and the cytoplasm serves multiple biological functions. To gain a biochemical understanding of the mechanisms for tRNA subcellular dynamics, we developed in vivo β-importin complex coimmunoprecipitation (co-IP) assays using budding yeast. Our studies provide the first in vivo biochemical evidence that two(More)
The ORF45 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a multifunctional tegument protein. Here, we characterize the transcriptional control of the ORF45 gene and show that its promoter can be activated by ORF50 protein, a latent-lytic switch transactivator. The ORF45 promoter can also be induced by sodium butyrate (SB), a histone(More)