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were first optimized for high level protein expression in E. coli. They were then synthesized using DNA oligo based, PCR gene assembling method. Poly-arginine tag plus a linker sequence, i.e. ESGGGGSPGRRRRRRRRRRR, was added to each protein C-terminal. The final DNA fragment was flanked with Nde I and Xho I sites, and inserted into pET41a expression vector(More)
Here we show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. With as little as 4 days of transgenic expression of these factors, mouse embryonic fibroblasts (MEFs) can be directly reprogrammed to spontaneously(More)
Saiyong Zhu,1 Wenlin Li,1 Hongyan Zhou,1 Wanguo Wei,1 Rajesh Ambasudhan,1 Tongxiang Lin,1 Janghwan Kim,1 Kang Zhang,2 and Sheng Ding1,* 1Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA 2Department of Ophthalmology and Shiley Eye Center, and Institute for Genomic Medicine, University of(More)
This paper explores two classes of model adaptation methods for Web search ranking: Model Interpolation and error-driven learning approaches based on a boosting algorithm. The results show that model interpolation, though simple, achieves the best results on all the open test sets where the test data is very different from the training data. The tree-based(More)
Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. Here, we report that a specific glycogen synthase kinase 3 (GSK-3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. When combined with Parnate (also named(More)
Parkinson's disease (PD) has been attributed to a combination of genetic and nongenetic factors. We studied a set of monozygotic twins harboring the heterozygous glucocerebrosidase mutation (GBA N370S) but clinically discordant for PD. We applied induced pluripotent stem cell (iPSC) technology for PD disease modeling using the twins' fibroblasts to evaluate(More)
Small ubiquitin-like modifier 4 (SUMO4) has been shown to have the potential to down-regulate NF-kappaB signal, leading to decreased transcription of pro-inflammatory cytokines. Recently, SUMO4 polymorphisms have been shown to be associated with several autoimmune diseases. In the present study, the association of SUMO4 polymorphisms with Behcet's disease(More)
PURPOSE IL-23 has been shown to be involved in the pathogenesis of Behcet's disease (BD) through promoting IL-17 production. This study examined whether IL-23R polymorphisms were associated with susceptibility to this disease in a Chinese Han population. METHODS Four single-nucleotide polymorphisms (SNP), rs7517847, rs11209032, rs 1343151 and rs17375018(More)
PURPOSE Corneal fibroblasts contribute to collagen remodeling in the corneal stroma in part by mediating collagen degradation. Given that corneal structure is influenced by sex hormone status, we examined the effects of sex hormones on collagen degradation by corneal fibroblasts. METHODS Rabbit corneal fibroblasts were cultured in three-dimensional(More)
OBJECTIVE To investigate the clinical features, causes of blindness and diagnosis of Vogt-Koyanagi-Harada (VKH) syndrome. METHODS The data of 157 patients with VKH syndrome were reviewed and analyzed. Patients were carefully examined with slit lamp, ophthalmoscope, three-mirror lens, fundus fluorescein angiography (FFA), indocyanine green angiography(More)