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were first optimized for high level protein expression in E. coli. They were then synthesized using DNA oligo based, PCR gene assembling method. Poly-arginine tag plus a linker sequence, i.e. ESGGGGSPGRRRRRRRRRRR, was added to each protein C-terminal. The final DNA fragment was flanked with Nde I and Xho I sites, and inserted into pET41a expression vector(More)
This paper explores two classes of model adaptation methods for Web search ranking: Model Interpolation and error-driven learning approaches based on a boosting algorithm. The results show that model interpolation, though simple, achieves the best results on all the open test sets where the test data is very different from the training data. The tree-based(More)
Here we show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. With as little as 4 days of transgenic expression of these factors, mouse embryonic fibroblasts (MEFs) can be directly reprogrammed to spontaneously(More)
Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. Here, we report that a specific glycogen synthase kinase 3 (GSK-3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. When combined with Parnate (also named(More)
PURPOSE IL-23 has been shown to be involved in the pathogenesis of Behcet's disease (BD) through promoting IL-17 production. This study examined whether IL-23R polymorphisms were associated with susceptibility to this disease in a Chinese Han population. METHODS Four single-nucleotide polymorphisms (SNP), rs7517847, rs11209032, rs 1343151 and rs17375018(More)
PURPOSE The aim of the study was to investigate the association of polymorphisms of the interleukin-23 receptor (IL23R) gene with Fuchs' syndrome in a Chinese Han population. METHODS Three single-nucleotide polymorphisms (SNPs), rs7517847, rs11209032 and rs17375018 of IL23R were genotyped in 138 Chinese Han patients with Fuchs' syndrome and 407 healthy(More)
Parkinson's disease (PD) has been attributed to a combination of genetic and nongenetic factors. We studied a set of monozygotic twins harboring the heterozygous glucocerebrosidase mutation (GBA N370S) but clinically discordant for PD. We applied induced pluripotent stem cell (iPSC) technology for PD disease modeling using the twins' fibroblasts to evaluate(More)
PURPOSE To address the clinical pattern and characteristics of uveitis in a tertiary center for uveitis in China and compare the similarity and difference in the distribution of uveitis entities between China and other countries. METHODS A retrospective study was performed on the patients with uveitis referred to the Zhongshan Ophthalmic Center from(More)