Hongtao Qiu

  • Citations Per Year
Learn More
To investigate the infection and replication of hepatitis B virus (HBV) in primary cultured human granulosa cells. Human granulosa cells were cultured with HBV-positive serum. Media were collected and assayed for HBsAg and HBeAg by ELISA, and HBV DNA by quantitative PCR. HBsAg and HBcAg were detected by immunocytochemistry in cultured cells. HBV DNA and RNA(More)
BACKGROUND/AIM The aim of this study was to investigate the factors that influence hepatitis B virus (HBV) expression and replication in the ovum. MATERIALS AND METHODS Immunohistochemistry and in situ hybridization techniques were used to assay the distributions of HBcAg, HBV DNA, and HBV mRNA in ovarian tissues and the ovum in 50 patients with chronic(More)
  • 1