Hongmei Yin

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PURPOSE Inappropriate contact lens (CL) use and care often lead to corneal neovascularization (corneal NV). We used mouse eyes which wore CL as the animal model to assess the reason for corneal NV with CL wear. The similar and overlapping activity of vascular endothelial growth factor (VEGF) and the potent angiogenic hypoxia-inducible factor 1α (HIF-1α)(More)
PURPOSE To determine the effectiveness of multigene-based anti-angiogenic gene therapies for experimental murine corneal neovascularization (corneal NV). METHODS Recombinant retroviral vectors encoding murine endostatin (mEndo), murine-soluble vascular endothelial growth factor receptor-2 (msFlk-1), or murine-soluble Tie2 (msTie2) were constructed and(More)
PURPOSE To investigate the expression of p16(INK4a), p21(WAF1/CIP1), p27(KIP1), and p53 in human corneal endothelial cell (HCEC) senescence ex vivo at various donor ages. METHODS Residual corneal tissues obtained after penetrating keratoplasty were used in this study. Age, death-to-preservation interval, and preservation-to-surgery interval of the donors(More)
PURPOSE The aim of this study was to investigate the expression and regulation of the four major inflammatory cytokines in fungal keratitis (FK) with the goal of further understanding its pathogenesis in order to develop more effective therapeutic approaches. METHODS Aspergillus fumigatus and Candida albicans were the corneal pathogens selected for this(More)
OBJECTIVE To examine the effect of recombinant canstatin protein on the corneal neovascularization (CorNV) in an alkaline burn-induced CorNV model. METHODS This study involved 50 C57BL/6 mice. CorNV was induced by an alkaline burn of the corneas with 1 N NaOH under general anesthesia. Beginning 24 h after CorNV induction, recombinant canstatin protein was(More)
To study the effects of nicotine on retinal pigment epithelium (RPE) cells in vivo and in vitro, (Balb/c x C57Bl/6) F1 mice were given water containing 100 microg/mL nicotine for six months. Cultured fetal RPE cells were treated with nicotine or lipopolysaccharide for seventy-two hours. Expression of matrix metalloproteinase protein (MMP)2, MMP9, and VEGF(More)
This study aimed to investigate the dynamic expression of glial fibrillary acidic protein (GFAP), a common neural factor, in cornea and stromal cells during herpes simplex virus-1 (HSV-1) infection. For each anesthetized BALB/c mouse, the cornea in one eye was inoculated with 1 × 10(5) plaque forming unit (PFU) of HSV-1, while the contralateral cornea was(More)
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