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The mitochondrion of Leishmania tarentolae contains approximately 35-40 tRNAs many of which comigrate with cytoplasmic tRNAs. Both mitochondrial (KtRNA) and cytoplasmic (CtRNA) tRNAs are functional, as they could be acylated either by mitochondrial or cytoplasmic synthetase extracts. There are two methionyl tRNA species in the cytoplasmic and mitochondrial(More)
Crithidia fasciculata cells grown in defined medium are sensitive to methotrexate (MTX), an inhibitor of dihydrofolate reductase (DHFR). When cells are challenged with 2-5 microM MTX, cell division ceases after 3-4 divisions and the cells become rounded and immotile for approximately 60 h, with a 40% decrease in cell viability occurring during this period.(More)
The aim of the present work was to quantify the passage of bovine immunoglobulins into the hemolymph of the tick Boophilus microplus during the feeding process and to determine their antibody activity. The knowledge is of paramount importance when vector control or blocking of disease transmission is attempted by vaccination of cattle. Approximately 2% of(More)
Babesia bovis is an intraerythrocytic protozoan that causes bovine babesiosis. Agarose gel electrophoresis of nucleic acids extracted from two isolates of B. bovis reveals, besides bulk DNA, an ethidium bromide-stainable band at about 5.5 kb. Further characterization of the latter with DNase I, RNase and mung bean nuclease suggested it to be a(More)
The major components of protein extracts from the cattle tick Boophilus microplus eggs and larvae of various ages were characterized by molecular sieving chromatography, ion exchange chromatography and SDS-PAGE. The fractions analysed showed a changing chromatographic pattern development. A serum raised against the components of a fraction showing(More)
In case studies of two herds of dairy cattle, a herd of 270 Friesian cows and heifers and a herd of 256 Jersey cows and heifers experienced high death rates and a protracted course of illness when fly ash was broadcast as a fertiliser over the farms at a rate of about 1 tonne per hectare. The onset of weakness, lassitude and diarrhoea in the cattle first(More)
A limited field study of covered facilities used for raising dairy calves suggested that respiratory disorders and death rates were highest when calves were continuously housed on bedding composed of wood shavings, where ventilation was poor and where automatic feeders were installed. High concentrations of ammonia were found in the urine-soaked bedding(More)
A case of selenium poisoning was suspected following the administration of 325 micromol selenium a day as sodium selenate (25 mg Se) for five consecutive days. The main signs were confined to changes in the integument which were loss of hair from the mane and tail, disintegration of the skin of the anus, lips, prepuce and scrotum, and separation of the(More)