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Many species in one: DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified
TLDR
It is demonstrated that the presence of COI numts makes this goal difficult to achieve when numts are prevalent and can introduce serious ambiguity into DNA barcoding, which strives for rapid and inexpensive generation of molecular species tags.
300 million years of diversification: elucidating the patterns of orthopteran evolution based on comprehensive taxon and gene sampling
TLDR
A robust phylogeny of Orthoptera is established including 36 of 40 families representing all 15 currently recognized superfamilies and based on complete mitochondrial genomes and four nuclear loci, in order to test previous phylogenetic hypotheses and to provide a framework for a natural classification and a reference for studying the pattern of divergence and diversification.
Nonstationary evolution and compositional heterogeneity in beetle mitochondrial phylogenomics.
TLDR
A broad range of phylogenetic methods are applied, including some traditional stationary models of evolution and all the more recent nonstationary models, and some of the newer software packages are found more appropriate for data of this nature.
A mitochondrial genome phylogeny of the Neuropterida (lace‐wings, alderflies and snakeflies) and their relationship to the other holometabolous insect orders
TLDR
Properly analysed, the mt genomic data set presented here is among the first molecular data to support traditional, morphology‐based interpretations of relationships between the three neuropterid orders and their grouping with Coleoptera.
Assessing the effects of primer specificity on eliminating numt coamplification in DNA barcoding: a case study from Orthoptera (Arthropoda: Insecta)
TLDR
The taxonomic distribution of numts is investigated by analysing cloned COI sequences and the effects of primer specificity on eliminating numt coamplification in four lineages are tested, which suggests that numts may be widespread in other taxonomic groups as well.
Mitochondrial genomics in Orthoptera using MOSAS
TLDR
It is hypothesized that the tRNAAsp–tRNALys rearrangement is a synapomorphy for Acridomorpha, but not Caelifera, and MOSAS, user-friendly software developed to facilitate annotation and analysis, is described.
Comparison of phylogenetic signal between male genitalia and non‐genital characters in insect systematics
TLDR
It is argued that the fact that male genitalia are under sexual selection and thus diverge rapidly does not necessarily equate with the lack of phylogenetic signal, because characters that evolve by descent with modification make appropriate characters for a phylogenetic analysis, despite the rate of evolution.
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