Hitoshi Nakayashiki

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Magnaporthe oryzae is the causal agent of rice blast disease, a devastating problem worldwide. This fungus has caused breakdown of resistance conferred by newly developed commercial cultivars. To address how the rice blast fungus adapts itself to new resistance genes so quickly, we examined chromosomal locations of AVR-Pita, a subtelomeric gene family(More)
Here we report the genetic analyses of histone lysine methyltransferase (KMT) genes in the phytopathogenic fungus Magnaporthe oryzae. Eight putative M. oryzae KMT genes were targeted for gene disruption by homologous recombination. Phenotypic assays revealed that the eight KMTs were involved in various infection processes at varying degrees. Moset1(More)
The LTR-retrotransposon MAGGY was introduced into naive genomes of Magnaporthe oryzae with different genetic backgrounds (wild-type, and MoDcl1 [mdl1] and MoDcl2 [mdl2] dicer mutants). The MoDcl2 mutants deficient in MAGGY siRNA biogenesis generally showed greater MAGGY mRNA accumulation and more rapid increase in MAGGY copy number than did the wild-type(More)
The significance of AVR1-CO39, an avirulence gene of the blast fungus corresponding to Pi-CO39(t) in rice cultivars, during the evolution and differentiation of the blast fungus was evaluated by studying its function and distribution in Pyricularia spp. When the presence or absence of AVR1-CO39 was plotted on a dendrogram constructed from ribosomal DNA(More)
Pseudomonas cichorii harbors the hrp genes. hrp-mutants lose their virulence on eggplant but not on lettuce. A phosphinothricin N-acetyltransferase gene (pat) is located between hrpL and an aldehyde dehydrogenase gene (aldH) in the genome of P. cichorii. Comparison of nucleotide sequences and composition of the genes among pseudomonads suggests a common(More)
Here we addressed the involvement of histone methylation at H3 lysine 4 (H3K4me) in substrate-dependent activation of cell-wall-degrading enzymes (CWDEs) in Magnaporthe oryzae. Transcriptional activation of 36 putative CWDEs including cellobiohydrolases, endoglucanases, β-glucosidases, and endoxylanases was assessed by qRT-PCR. Among the CWDE genes, 16 were(More)
The gene list under the H3K4me2 column on the enriched_in_GT tab in S3 Table is incorrect. Please view the correct S3 Table below. S3 Table. List of genes differentially enriched for H3K4me2/me3 between mycelia and germtubes. access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution,(More)
Hyphal interactions of 10 Rosellinia necatrix isolates were analyzed. Most incompatible pairings produced broad melanized barrage lines, with a few exceptions. The variation in barrage lines revealed using light microscopy might result from distinct frequencies of different hyphal interaction types. In incompatible pairings, hyphal anastomosis was rarely(More)
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