Hisayuki Hashimoto

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Tomohisa Seki,1,7 Shinsuke Yuasa,1,2,7 Mayumi Oda,2 Toru Egashira,1 Kojiro Yae,1 Dai Kusumoto,1 Hikari Nakata,1 Shugo Tohyama,1 Hisayuki Hashimoto,1 Masaki Kodaira,1 Yohei Okada,2,3 Hiroyuki Seimiya,4 Noemi Fusaki,5,6 Mamoru Hasegawa,5 and Keiichi Fukuda1,* 1Department of Cardiology 2Center for Integrated Medical Research 3Department of Physiology Keio(More)
Heart disease remains a major cause of death despite advances in medical technology. Heart-regenerative therapy that uses pluripotent stem cells (PSCs) is a potentially promising strategy for patients with heart disease, but the inability to generate highly purified cardiomyocytes in sufficient quantities has been a barrier to realizing this potential.(More)
Fibroblasts can be directly reprogrammed into cardiomyocyte-like cells (iCMs) by overexpression of cardiac transcription factors or microRNAs. However, induction of functional cardiomyocytes is inefficient, and molecular mechanisms of direct reprogramming remain undefined. Here, we demonstrate that addition of miR-133a (miR-133) to Gata4, Mef2c, and Tbx5(More)
AIMS Long QT syndrome (LQTS) is an inheritable and life-threatening disease; however, it is often difficult to determine disease characteristics in sporadic cases with novel mutations, and more precise analysis is necessary for the successful development of evidence-based clinical therapies. This study thus sought to better characterize ion channel cardiac(More)
Duchenne muscular dystrophy (DMD) is a chronic and life-threatening disease that is initially supported by muscle regeneration but eventually shows satellite cell exhaustion and muscular dysfunction. The life-long maintenance of skeletal muscle homoeostasis requires the satellite stem cell pool to be preserved. Asymmetric cell division plays a pivotal role(More)
We previously reported a cytosolic pyruvate kinase (EC from Toxoplasma gondii (TgPyKI) that differs from most eukaryotic pyruvate kinases in being regulated by glucose 6-phosphate rather than fructose 1,6-diphosphate. Another putative pyruvate kinase (TgPyKII) was identified from parasite genome, which exhibits 32% amino acid sequence identity to(More)
We examined guanosine triphosphate (GTP)-binding proteins (G-proteins) in synovial tissues obtained from patients with rheumatoid arthritis or osteoarthritis. The results of immunoblot analysis with anti-bovine G-protein betagamma-subunit antibody in the rheumatoid synovial tissue were similar to those in the osteoarthritis synovial tissue. On the other(More)
RATIONALE The transcriptional networks guiding heart development remain poorly understood, despite the identification of several essential cardiac transcription factors. OBJECTIVE To isolate novel cardiac transcription factors, we performed gene chip analysis and found that Zac1, a zinc finger-type transcription factor, was strongly expressed in the(More)
MicroRNAs (miRNAs) play a pivotal role during embryonic development and are required for proper organogenesis, including hematopoiesis. Recent studies suggest that, in the early mesoderm, there is an interaction between the hematopoietic and cardiac lineages. However, whether miRNAs can affect other lineages remains unknown. Therefore, we investigated(More)
Mammalian cardiomyocytes withdraw from the cell cycle shortly after birth, although it remains unclear how cardiomyocyte cell cycles behave during development. Compared to conventional immunohistochemistry in static observation, time-lapse imaging can reveal comprehensive data in hard-to-understand biological phenomenon. However, there are no reports of an(More)