Hisayoshi Ooka

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This study was undertaken to determine whether and how advanced glycation end products (AGE), senescent macroproteins accumulated in various tissues under hyperglycemic states, cause angiogenesis, the principal vascular derangement in diabetic microangiopathy. We first prepared AGE-bovine serum albumin (BSA) and anti-AGE antiserum using AGE-RNase A. Then(More)
Purpose: Recent studies in humans and mice have demonstrated that intestinal P-glycoprotein plays a causative role in the limited absorption of orally administered paclitaxel. Multidrug resistance (MDR)-reversing agents, such as cyclosporin A and PSC 833, are known to increase the systemic exposure to orally administered paclitaxel by enhancing absorption(More)
We studied changes in the distribution pattern of relative RNA content during the in vitro aging of TIG-3 cells by flow cytometry (FACS III). Propidium iodide (PI) does not stain total cellular RNA, but it intercalates specifically into double-helical regions of both DNA and RNA. In applying this principle to RNA, we stained double-stranded RNA (dsRNA) in(More)
The electrophoretic mobility of human diploid fibroblasts, TIG-1, was studied at different passages. The net negative surface charge of the cells decreased from -1.658 +/- 0.108 micron/s/V/cm at an early passage (15 population doublings, PD) to -1.173 +/- 0.116 at the final passage (67 PD) in 1/15 M phosphate buffer supplemented with 5.4% glucose. The(More)
Wistar rats were made hypothyroidic by intraperitoneal thyroxine (T4) injection during the first 10 days of neonatal life. Levels of T4, 3,3',5-triiodothyronine (T3), thyroid-stimulating hormone (TSH) and prolactin in the blood of these rats were measured by radioimmunoassay. The T4 levels are about two-thirds of control values up to 20 months of age. T3(More)
A new human diploid cell strain, TIG-7, which has the male karyotype, was established and characterized. Isozyme and histocompatibility typing of the cell strain was performed. The average in vitro life span of the cells is 73 population doublings. Changes in cell volume, doubling time, saturation density, the efficiency of cell attachment, plating(More)
Age-related changes in the numbers of mammotrophs, somatotrophs, mammosomatotrophs, and the cells of other types in the anterior pituitary of female Wistar rat were measured by flow cytometry. The mammotrophs increased with age, and the somatotrophs decreased with senescence. The mammosomatotrophs increased remarkably in senescent rats, and these cells of(More)