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We developed a novel technique to manipulate lipid nanotubes in 3D by laser tweezers in water solutions. Laser tweezers are well known as one of the noncontact manipulation methods used in a closed space. We reported on 3D 6DOF manipulation of microbeads using 3D Synchronized Laser Micromanipulation (SLM), however, the blur is generated in the microscope(More)
In noncontact cell manipulation with optical tweezers, we need to use microtools to avoid damages to the cells by direct laser irradiation. When we manipulate complex structures such as microtools, a multi-beam optical tweezers which can manipulate multiple objects is suitable; however manipulation speed or trapping number are not so good. Therefore, we(More)
A major problem for analysis of bio-nanoparticles such as the influenza viruses (size is about 100 nm) is that sample concentration is low. We developed manipulation of the single virus using optical tweezers supported by dielectrophoretic concentration of the viruses in a microfluidic chip. The microfluidic chip made of poly (dimethyl siloxane) (PDMS) is(More)
Production of functional microtools having an arbitrary shape by self-assembly of microparticles and heat treatment above the glass transition temperature of the microparticles was developed. Polystyrene microbeads were used as a material of the microtool. A solution including microparticles was dispersed onto the silicon substrate having microtool patterns(More)
Optical adhesion control of a functional gel microtool, made of hydrophilic photo-crosslinkable resin, was developed for on-chip cell manipulation. The functional gel microtool used can be manipulated by optical tweezers and functionalized by spiropyran chromospheres, which is a photochromic polymer. Spiropyran chromospheres were used for temporal cell(More)
This paper demonstrates local pH measurement in a microchip using a pH-sensing gel-microbead. To achieve this, the gel-microbead made of a hydrophilic photo-crosslinkable resin was functionalized with the pH indicator bromothymol blue (BTB). The primary constituent of this photo-crosslinkable resin is poly(ethylene glycol). Gel-microbeads impregnated with(More)
A local pH measurement method in a microchip using a functional gel-tool was developed. We used salting-out gel-tools impregnated with Bromothymol Blue (BTB), which is a pH indicator. The gel-tool is made of hydrophilic photo-crosslinkable resin. The primary constituent of this photo-crosslinkable resin is polyethyleneglycol. A solution mixed with(More)
Handling of microorganisms is of great interest in biology and biotechnology. Single cell capture technology has been demonstrated in our previous work with thermal gelation material and micro fluidic devise. This paper extends the system by local flow rate control to stabilize the trap with in-situ fabrication by photo-crosslinkable resin. Target cells are(More)