Hisataka Maruyama

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In noncontact cell manipulation with optical tweezers, we need to use microtools to avoid damages to the cells by direct laser irradiation. When we manipulate complex structures such as microtools, a multi-beam optical tweezers which can manipulate multiple objects is suitable; however manipulation speed or trapping number are not so good. Therefore, we(More)
A novel approach appropriate for rapid separation and immobilization of a single cell by concomitantly utilizing laser manipulation and locally thermosensitive hydrogelation is proposed in this paper. We employed a single laser beam as optical tweezers for separating a target cell and locating it adjacent to a fabricated, transparent micro heater.(More)
A novel separation method for random screening of target cells from a large heterogeneous population by using a local photo-polymerization is developed. A photo-crosslinkable resin solution is mixed with the sample liquid and we controlled the state from sol to gel by irradiating the near ultraviolet (UV) light with the mercury lamp and He-Cd laser near the(More)
We developed photo-induced selective injection of nanotool into a specific cell for intracellular measurement in a microfluidic chip. We used membrane fusion of the liposome for invasive cell injection of the nanotool. The nanotool was modified by photo-responsive chemical for pH control and fluorescent dye for environment measurement. The nanotool was(More)
A major problem for analysis of bio-nanoparticles such as the influenza viruses (size is about 100 nm) is that sample concentration is low. We developed manipulation of the single virus using optical tweezers supported by dielectrophoretic concentration of the viruses in a microfluidic chip. The microfluidic chip made of poly (dimethyl siloxane) (PDMS) is(More)
Photoautotrophic bacteria have developed mechanisms to maintain K(+) homeostasis under conditions of changing ionic concentrations in the environment. Synechocystis sp. strain PCC 6803 contains genes encoding a well-characterized Ktr-type K(+) uptake transporter (Ktr) and a putative ATP-dependent transporter specific for K(+) (Kdp). The contributions of(More)
Production of functional microtools having an arbitrary shape by self-assembly of microparticles and heat treatment above the glass transition temperature of the microparticles was developed. Polystyrene microbeads were used as a material of the microtool. A solution including microparticles was dispersed onto the silicon substrate having microtool patterns(More)
This paper demonstrates local pH measurement in a microchip using a pH-sensing gel-microbead. To achieve this, the gel-microbead made of a hydrophilic photo-crosslinkable resin was functionalized with the pH indicator bromothymol blue (BTB). The primary constituent of this photo-crosslinkable resin is poly(ethylene glycol). Gel-microbeads impregnated with(More)
We developed a novel technique to manipulate lipid nanotubes in 3D by laser tweezers in water solutions. Laser tweezers are well known as one of the noncontact manipulation methods used in a closed space. We reported on 3D 6DOF manipulation of microbeads using 3D Synchronized Laser Micromanipulation (SLM), however, the blur is generated in the microscope(More)