Hisashi Chuda

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In our previous studies, we tentatively identified 17,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) as a maturation-inducing hormone (MIH) in yellowtail (Seriola quinqueradiata) through in vivo and in vitro experiments. In this study, we investigated the binding sites for radioactive 17,20 beta-P and characterized the receptor binding to the ovarian(More)
Final oocyte maturation and ovulation of captive chub mackerel Scomber japonicus with fully yolk-accumulated occytes were induced by a single injection of human chorionic gonadotropin. Reproductive parameters, including spawning frequency and batch fecundity, which are required to estimate spawning biomass in pelagic fish by the daily egg production method,(More)
To study the physiological roles of gonadotropins (GtHs) in the yellowtail, the cDNAs encoding each GtH subunit (GPHα, FSHβ and LHβ) and their receptors (FSHR and LHR) were isolated from the pituitary gland and gonads using the polymerase chain reaction (PCR). In addition, thyrotropin (TSH) and its receptor (TSHR) cDNAs, were isolated from the pituitary(More)
The water flow in larval rearing tanks has been indicated to cause mass mortality of the seven-band grouper Epinephelus septemfasciatus larvae. Therefore, a new aerating method was tested in an actual scale intensive rearing tank (8.0 m in diameter, 1.87 m of water depth, 100 m3 of volume), in which an aerator was positioned at the center of the rearing(More)
The cDNA for glycoprotein hormone (GPH) α-subunit, β-subunits of the FSH (GtH I) and LH (GtH II) were isolated, cloned, and sequenced from sevenband grouper pituitary using RACE PCR. These results show that sevenband grouper has two different types of gonadotropins FSH (GtH I) and LH (GtH II), as other teleosts.
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