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We describe Trans-ABySS, a de novo short-read transcriptome assembly and analysis pipeline that addresses variation in local read densities by assembling read substrings with varying stringencies and then merging the resulting contigs before analysis. Analyzing 7.4 gigabases of 50-base-pair paired-end Illumina reads from an adult mouse liver poly(A) RNA(More)
Using DNA sequences 5' to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expressing wild-type animals were analyzed at(More)
Array comparative genomic hybridization (aCGH) has been used primarily to detect copy-number variants between two genomes. Here we report using aCGH to detect single nucleotide mutations on oligonucleotide microarrays with overlapping 50-mer probes. This technique represents a powerful method for rapidly detecting novel homozygous single nucleotide(More)
This study is one of the few attempts to investigate customers' intention to repurchase in Internet shopping. Using an online questionnaire filled out by 1,111 online customers to conduct a multiple regression analysis, a new factor, perceived benefits, had a positive impact on the repurchase intention. In addition, extrinsic benefits measured by time and(More)
The goal of fast adaptations and modifications according to continuous change requests is common to all software engineering methodologies. In most cases, sooner or later this results in a mismatch between the software specification and the actual implementation with related disadvantages such as the impossibility of performing validations of related(More)
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